Mammalian cell genetics. I. Selection and characterization of mutations auxotrophic for L-glutamine or resistant to 8-azaguanine in Chinese hamster cells in vitro.

Genetics ·Vol. 62 ·No. 2 ·1969-06-00 ·Pages 359-77

Chu EH, Brimer P, Jacobson KB, Merriam EV

Abstract

暂无摘要

MeSH Terms

Animals Azaguanine/pharmacology Cell Line Clone Cells Cricetinae Culture Techniques Genetics Glutamine/metabolism Hybridization, Genetic Hypoxanthines/metabolism Karyotyping Male Mutation Selection, Genetic Transferases/metabolism

Chemicals

Hypoxanthines Glutamine Transferases Azaguanine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Chu E H

Brimer P

Jacobson K B

Merriam E V

References (11)

11 references, click to expand

A method of selecting for auxotrophic mutants of HeLa cells.
J Exp Med. 1960 Apr 1;111:559-72 PMID: 13720702
Measurement of cell growth in tissue culture with a phenol reagent (folin-ciocalteau).
Proc Soc Exp Biol Med. 1956 Feb;91(2):305-7 PMID: 13297784
DIFFERENTIATION IN MONOLAYER TISSUE CULTURE CELLS.
Adv Genet. 1964;12:143-280 PMID: 14278624
Hybridization of a malignant melanoma cell line with L cells in vitro.
Cancer Res. 1967 Nov;27(11):1953-60 PMID: 6073493
ESTIMATION OF MUTATION RATES WITH MAMMALIAN CELLS IN CULTURE.
Proc Natl Acad Sci U S A. 1959 Jun;45(6):872-7 PMID: 16590462
Loss of T-antigen from somatic hybrids between mouse cells and SV40-transformed human cells.
Proc Natl Acad Sci U S A. 1968 Apr;59(4):1132-5 PMID: 4297332
THE INOSINIC ACID PYROPHOSPHORYLASE ACTIVITY OF MOUSE FIBROBLASTS PARTIALLY RESISTANT TO 8-AZAGUANINE.
Proc Natl Acad Sci U S A. 1963 Sep;50:568-73 PMID: 14067106
Mutations of Bacteria from Virus Sensitivity to Virus Resistance.
Genetics. 1943 Nov;28(6):491-511 PMID: 17247100
Genetics of somatic mammalian cells. V. Treatment with 5-bromodeoxyuridine and visible light for isolation of nutritionally deficient mutants.
Proc Natl Acad Sci U S A. 1967 Sep;58(3):1227-34 PMID: 5233843
Enzyme defect associated with a sex-linked human neurological disorder and excessive purine synthesis.
Science. 1967 Mar 31;155(3770):1682-4 PMID: 6020292
A convenient method for assay of DNA synthesis in synchronized human cell cultures.
J Cell Biol. 1966 Jan;28(1):139-43 PMID: 5901496

Article Info

Journal

Genetics

Abbr.

Genetics

ISSN

0016-6731

DOI

10.1093/genetics/62.2.359

Published

1969-06-00

Pages

359-77

Language

English

Region

United States

NLM ID

0374636

PMCID

PMC1212281

Subset

External Links

PubMed source DOI: 10.1093/genetics/62.2.359 PMC Full Text View journal details

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Mammalian cell genetics. I. Selection and characterization of mutations auxotrophic for L-glutamine or resistant to 8-azaguanine in Chinese hamster cells in vitro.

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