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PMID: 5411079 Published · ppublish English Journal Article

Evidence for variation in the quantity of DNA among plastids of Acetabularia.

The Journal of cell biology ·Vol. 44 ·No. 2 ·1970-02-00 ·Pages 361-75

Woodcock CL, Bogorad L

Abstract

The DNA content of individual plastids of the giant unicellular algae Acetabularia mediterranea, and Polyphysa cliftoni was studied. Four methods were used for localizing DNA: acridine orange staining, radioautography following actinomycin D-(3)H treatment, electron microscopy of thin tissue sections, and electron microscopy of osomotically disrupted plastids. With each method, DNA was readily detected in 20-35% of plastids, but no DNA was observed in the remaining 65-80%. The results further showed that in those plastids with detectable DNA the amount of DNA present was variable. The sensitivity and reliability of the localization methods are discussed, and the possible implications of these findings are considered.

MeSH Terms
Autoradiography Binding Sites Chlorophyta/analysis Chloroplasts/analysis DNA/analysis Dactinomycin Microscopy, Electron Microscopy, Fluorescence Osmosis Tritium
Chemicals
Tritium Dactinomycin DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Woodcock C L
Bogorad L
References (25)
25 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.44.2.361
Published
1970-02-00
Pages
361-75
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2107954
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.44.2.361 PMC Full Text View journal details
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