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PMID: 5427117 Published · ppublish English Journal Article

The effect of 3-(3,4-dichlorophenyl)-1,1-dimethylurea on chloroplast development and maintenance in Euglena gracilis. I. Ultrastructural characterization of light-grown cells by the techniques of thin sectioning and freeze-etching.

Plant physiology ·Vol. 45 ·No. 4 ·1970-04-00 ·Pages 475-83

Holt SC, Stern AI

Abstract

The ultrastructure of light-grown Euglena gracilis var. bacillaris was examined by the techniques of thin sectioning and freeze-etching. Thin sectioning revealed the typical organelles previously observed in chemically fixed Euglena. In addition to confirming the observations on thin sections, the freeze-etch technique has revealed the presence in E. gracilis of a complex multilaminar pellicle, and an ordered arrangement to the paramylon granule. The chloroplast thylakoids are particulate and similar to those observed in higher plants.

MeSH Terms
Chloroplasts/drug effects Euglena/cytology Freeze Etching Herbicides/pharmacology Microscopy, Electron Polysaccharides Urea/pharmacology
Chemicals
Herbicides Polysaccharides Urea
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Holt S C
Stern A I
References (21)
21 references, click to expand
  1. Current models for the structure of biological membranes.
    J Cell Biol. 1969 Sep;42(3):613-46 PMID: 4895596
  2. [FREEZE-FIXATION OF LIVING CELLS AND ITS USE IN ELECTRON MICROSCOPY].
    Z Zellforsch Mikrosk Anat. 1964 Apr 28;62:546-80 PMID: 14212694
  3. Use of freeze-etching in the study of biological ultrastructure.
    Int Rev Exp Pathol. 1966;5:179-216 PMID: 4877344
  4. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.
    Plant Physiol. 1949 Jan;24(1):1-15 PMID: 16654194
  5. A comparison of chloroplast membrane surfaces visualized by freeze-etch and negative staining techniques; and ultrastructural characterization of membrane fractions obtained from digitonin-treated spinach chloroplasts.
    J Cell Biol. 1969 Oct;43(1):16-31 PMID: 4186409
  6. Function of the "quantasome" in photosynthesis: structure and properties of membrane-bound particle active in the dark reactions of photophosphorylation.
    Proc Natl Acad Sci U S A. 1967 Sep;58(3):1261-8 PMID: 16578666
  7. Subunits in chloroplast lamellae.
    J Ultrastruct Res. 1967 Aug;19(3):283-303 PMID: 4167849
  8. Structure of the paramylon from Euglena gracilis.
    Biochim Biophys Acta. 1960 Oct 21;44:161-3 PMID: 13693876
  9. Electron microscopy of the cell envelope of Ferrobacillus ferrooxidans prepared by freeze-etching and chemical fixation techniques.
    J Bacteriol. 1966 Dec;92(6):1765-71 PMID: 5334768
  10. The continued presence of quantasomes in ethylenediaminetetraacetate-washed chloroplast lamellae.
    Proc Natl Acad Sci U S A. 1968 May;60(1):337-43 PMID: 4968635
  11. Observations on the fine structure of the pellicle pores of Euglena granulata.
    Protoplasma. 1967;64(3):330-44 PMID: 6081636
  12. The photosynthetic apparatus in chloroplasts of higher plants.
    Brookhaven Symp Biol. 1966;19:353-74 PMID: 5966916
  13. Studies of Chloroplast Development in Euglena. VII. Fine Structure of the Developing Plastid.
    Plant Physiol. 1964 Mar;39(2):231-40 PMID: 16655903
  14. Fine structure of Ectothiorhodospira mobilis Pelsh.
    J Bacteriol. 1968 Jun;95(6):2374-92 PMID: 5669908
  15. STRUCTURE AND PHYSIOLOGY OF EUGLENA SPIROGYRA. 3-6..
    Arch Mikrobiol. 1965 Jan 22;50:133-55 PMID: 14304664
  16. X-ray diagrams of Euglena-paramylon, of the acid-insoluble glucan of yeast cell walls and of laminarin.
    Biochim Biophys Acta. 1952 Dec;9(6):699-700 PMID: 13032182
  17. Fine structure of Ectothiorhodospira mobilis strain 8113 thylakoids: chemical fixation and freeze-etching studies.
    Arch Mikrobiol. 1968;62(2):111-28 PMID: 5711185
  18. Subunits in the membranes of chloroplasts of Phaseolus vulgaris, Pisum sativum, and Aspidistra sp.
    J Ultrastruct Res. 1965 Aug;13(1):92-111 PMID: 5841097
  19. ROLE OF LYSOSOMES IN CELLULAR LYTIC PROCESSES. I. EFFECT OF CARBON STARVATION IN EUGLENA GRACILIS.
    Exp Mol Pathol. 1964 Dec;90:583-609 PMID: 14243136
  20. Enzymatic properties of the inner and outer membranes of rat liver mitochondria.
    J Cell Biol. 1968 Jul;38(1):158-75 PMID: 5691970
  21. Comparative ultrastructure of selected aerobic spore-forming bacteria: a freeze-etching study.
    Bacteriol Rev. 1969 Jun;33(2):346-78 PMID: 4979698
Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
Published
1970-04-00
Pages
475-83
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC396435
Subset
IM
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