Home LiteratureArticle Details
PMID: 5438107 Published · ppublish English Journal Article

Process of infection with bacteriophage phiX174. 33. Templates for the synthesis of single-stranded deoxyribonucleic acid.

Journal of virology ·Vol. 5 ·No. 3 ·1970-03-00 ·Pages 282-8

Funk F, Sinsheimer RL

Abstract

The origin of the templates for the synthesis of phiX174 progeny single-stranded deoxyribonucleic acid was studied by means of the mutagenic activity associated with the decay of incorporated (3)H-labeled 5-cytosine. The results indicate that the single-strand synthesis occurs in an asymmetric semiconservative manner using as template the complementary strands of the pool of replicative from molecules accumulated during the eclipse period. These complementary strands are repeatedly used as templates, and there is no detectable preferential use of complementary strand templates made early in the eclipse versus those made late.

MeSH Terms
Bromodeoxyuridine/metabolism Coliphages/classification,metabolism Culture Media Cytosine/metabolism DNA, Viral/biosynthesis Genetics, Microbial Mutation Temperature Tritium Virus Replication
Chemicals
Culture Media DNA, Viral Tritium Cytosine Bromodeoxyuridine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Funk F
Sinsheimer R L
References (18)
18 references, click to expand
  1. IDENTIFICATION OF THE ALTERED BASES IN MUTATED SINGLE-STRANDED DNA. II. IN VIVO MUTAGENESIS BY 5-BROMODEOXYURIDINE AND 2-AMINOPURINE.
    J Mol Biol. 1964 Aug;9:364-71 PMID: 14202272
  2. IDENTIFICATION OF THE ALTERED BASES IN MUTATED SINGLE-STRANDED DNA. I. IN VITRO MUTAGENESIS BY HYDROXYLAMINE, ETHYL METHANESULFONATE AND NITROUS ACID.
    J Mol Biol. 1964 Aug;9:352-63 PMID: 14202271
  3. The process of infection with bacteriophage phi-X174. 3. Phage maturation and lysis after synchronized infection.
    J Mol Biol. 1965 Jul;12(3):641-6 PMID: 5323480
  4. The process of infection with bacteriophage phi-X174. IV. Replication of the viral DNA in a synchronized infection.
    J Mol Biol. 1965 Jul;12(3):647-62 PMID: 5323481
  5. Evidence for a mutagenic local effect accompanying the decay of incorporated tritium in Escherichia coli.
    J Mol Biol. 1965 Sep;13(2):600-2 PMID: 5325730
  6. The process of infection with bacteriophage phi-X174. IX. Studies on the physiology of three phi-X174 temperature-sensitive mutants.
    J Mol Biol. 1966 Apr;16(2):374-86 PMID: 5954170
  7. A determination of mutagen specificity in bacteria using nonsense mutants of bacteriophage T4.
    J Mol Biol. 1967 Jun 28;26(3):437-47 PMID: 6029739
  8. Some factors which influence the replication of the replicative form of bacteriophage OX174.
    Biochem Biophys Res Commun. 1967 Feb 8;26(3):247-54 PMID: 6034349
  9. Process of infection with bacteriophage phi-X174. XIV. Studies on macromolecular synthesis during infection with a lysis-defective mutant.
    J Mol Biol. 1967 Aug 28;28(1):87-94 PMID: 4860988
  10. The process of infection with bacteriophage phi-X174. XXII. Synthesis of progeny single-stranded DNA.
    Proc Natl Acad Sci U S A. 1968 Mar;59(3):911-6 PMID: 5238671
  11. The process of infection with bacteriophage phi X174. XVI. Synthesis of the replicative form and its relationship to viral single-stranded DNA synthesis.
    J Mol Biol. 1968 Mar 14;32(2):285-302 PMID: 4868423
  12. The process of infection with bacteriophage phi-X174. XXI. Replication and fate of the replicative form.
    Proc Natl Acad Sci U S A. 1968 Feb;59(2):577-81 PMID: 5238985
  13. Bacteriophage phi-X174 and related viruses.
    Prog Nucleic Acid Res Mol Biol. 1968;8:115-69 PMID: 4874231
  14. The conversion of amber suppressors to ochre suppressors.
    Proc Natl Acad Sci U S A. 1968 Jul;60(3):1030-7 PMID: 4875805
  15. The process of infection with bacteriophage phiX174. 8. Evidence for an essential bacterial "site".
    J Virol. 1967 Feb;1(1):135-44 PMID: 4918231
  16. Process of infection with bacteriophage phiX174. XX. Attachment of the parental DNA of bacteriophage phiX174 to a fast-sedimenting cell component.
    J Mol Biol. 1968 May 28;34(1):17-29 PMID: 5760452
  17. The process of infection with bacteriophage phi-XI74. I. Evidence for a "replicative form".
    J Mol Biol. 1962 Mar;4:142-60 PMID: 13913400
  18. DIFFERENTIAL MUTATION PRODUCTION BY THE DECAY OF INCORPORATED TRITIUM COMPOUNDS IN E. COLI.
    Biophys J. 1964 Sep;4:355-65 PMID: 14205506
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
Published
1970-03-00
Pages
282-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC376002
Subset
IM
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]