Abstract
Glucans were isolated from the cell wall of the yeast (Y) and mycelial (M) forms of Paracoccidioides brasiliensis. The alkali-soluble glucan of the Y form had properties of alpha-1,3-glucan. The alkali-insoluble glucan of the M form was identified as a beta-glucan which contains a beta-(1 --> 3)-glycosidic linkage by infrared absorption spectrum, by effect of beta-1,3-glucanase, and by partial acid hydrolysis. The alkali-soluble glucans of the M form were a mixture of alpha- and beta-glucans and the ratio of alpha- to beta-glucan was variable, depending on the preparations.
MeSH Terms
Absorption
Blastomyces/enzymology
Cell Wall/analysis
Cellulose/analysis
Chromatography, Paper
Chromatography, Thin Layer
Colorimetry
Fungi/analysis,enzymology
Glycoside Hydrolases/metabolism
Infrared Rays
Polymorphism, Genetic
Solubility
Spectrophotometry
Temperature
Chemicals
Cellulose
Glycoside Hydrolases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kanetsuna F
Carbonell L M
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14 references, click to expand
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