Histone changes during degeneration and regeneration of the pancreas.

The Biochemical journal ·Vol. 117 ·No. 4 ·1970-05-00 ·Pages 711-4

Fitzgerald PJ, Marsh WH, Ord MG, Stocken LA

Abstract

1. Changes in the microstructure of histones from the pancreas were followed in rats given ethionine while on a protein-free diet. The lysine-rich histone F1 and the other histones extracted subsequently with 250mm-hydrochloric acid were isolated. When the ethionine-treated rats returned to the stock diet, regeneration of the pancreas was accompanied by an immediate increase in phosphate content of the total histone extract from which histone F1 had been removed. After 3-4 days there was a further increase in the phosphate content of this extract, and also in that of histone F1. 2. Similar changes in the phosphate and thiol+disulphide content of pancreas histones other than F1 were produced by starvation and re-feeding.

MeSH Terms

Animals DNA/biosynthesis Ethionine Histones/analysis Male Pancreas/analysis,drug effects,metabolism Phosphates/analysis RNA/biosynthesis Rats Regeneration Starvation Thymidine/metabolism Tritium Uridine/metabolism

Chemicals

Histones Phosphates Tritium RNA DNA Thymidine Uridine Ethionine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Fitzgerald P J

Marsh W H

Ord M G

Stocken L A

References (11)

11 references, click to expand

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Phosphorylation of nuclear protein early in the course of gene activation in lymphocytes.
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Pancreatic acinar cell regeneration.
Nature. 1966 Nov 5;212(5062):594-6 PMID: 5971678
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Biochem J. 1969 Mar;112(1):81-9 PMID: 4180186
Pancreatic acinar cell regeneration. II. Enzymatic, nucleic acid, and protein changes.
Am J Pathol. 1968 May;52(5):1013-37 PMID: 5646509
Variations in the phosphate content and thiołdisulphide ratio of histones during the cell cycle. Studies with regenerating rat liver and sea urchins.
Biochem J. 1968 Apr;107(3):403-10 PMID: 5689839
The effect of ionizing radiation on nuclear phosphorylation in the radio-sensitive tissues of the rat.
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Biochem J. 1964 Jul;92(1):55-9 PMID: 5840387
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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1170711

Published

1970-05-00

Pages

711-4

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1179022

Subset

External Links

PubMed source DOI: 10.1042/bj1170711 PMC Full Text View journal details

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Histone changes during degeneration and regeneration of the pancreas.

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