Home LiteratureArticle Details
PMID: 5449179 Published · ppublish English Journal Article

Structure-function relationships in the adipose cell. II. Pinocytosis and factors influencing its activity in the isolated adipose cell.

The Journal of cell biology ·Vol. 46 ·No. 2 ·1970-08-00 ·Pages 342-53

Cushman SW

Abstract

Pinocytic activity in the adipose cell has been examined by measuring the uptake of colloidal gold. Pinocytic activity occurs in the isolated adipose cell under all experimental conditions; a portion of the vesicular elements of the cell can be identified by electron microscopy as pinocytic in origin. The isolated adipose cell appears to take up serum albumin by pinocytosis. Pinocytic activity in the isolated adipose cell is enhanced by epinephrine, but not by insulin. The relationship between pinocytosis and the metabolic activity of the adipose cell has been studied by measuring simultaneously the uptake of radioactive colloidal gold, the incorporation of (14)C-counts from U-glucose-(14)C into CO(2), total lipid, triglyceride glycerol and triglyceride fatty acids, and the release of nonesterified fatty acids in the absence of hormones and in the presence of insulin or epinephrine. Correlations between hormone-produced alterations in lipid metabolism and in pinocytic activity suggest that intracellular nonesterified fatty acid levels are a factor in the regulation of both the cell's pinocytic activity and its metabolism and that pinocytosis in the adipose cell functions in the extracellular-intracellular transport of nonesterified fatty acids.

MeSH Terms
Adipose Tissue/cytology,drug effects,metabolism Animals Carbon Dioxide/biosynthesis Carbon Isotopes Epinephrine/pharmacology Fatty Acids, Nonesterified/analysis Glucose/metabolism Gold Colloid, Radioactive/metabolism Insulin/pharmacology Lipids/analysis Male Microscopy, Electron Pinocytosis Rats Serum Albumin, Radio-Iodinated/metabolism Triglycerides/analysis
Chemicals
Carbon Isotopes Fatty Acids, Nonesterified Gold Colloid, Radioactive Insulin Lipids Serum Albumin, Radio-Iodinated Triglycerides Carbon Dioxide Glucose Epinephrine
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Cushman S W
References (27)
27 references, click to expand
  1. The in vitro differentiation of mononuclear phagocytes. 3. The reversibility of granule and hydrolytic enzyme formation and the turnover of granule constituents.
    J Exp Med. 1965 Sep 1;122(3):455-66 PMID: 5320303
  2. Effect of glucose on spontaneous limitation of lipolysis in isolated adipose tissue: a potential regulatory mechanism.
    Ann N Y Acad Sci. 1965 Oct 8;131(1):143-56 PMID: 4285773
  3. Modulation of lipolysis in adipose tissue by fatty acid concentration in fat cell.
    Ann N Y Acad Sci. 1965 Oct 8;131(1):302-14 PMID: 4285776
  4. Flux of palmitic acid across the adipose tissue membrane.
    Ann N Y Acad Sci. 1965 Oct 8;131(1):78-90 PMID: 5217002
  5. The in vitro differentiation of mononuclear phagocytes. V. The formation of macrophage lysosomes.
    J Exp Med. 1966 Apr 1;123(4):757-66 PMID: 5931922
  6. The regulation of pinocytosis in mouse macrophages. I. Metabolic requirements as defined by the use of inhibitors.
    J Exp Med. 1966 Oct 1;124(4):557-71 PMID: 5950886
  7. Glomerular permeability. Ultrastructural cytochemical studies using peroxidases as protein tracers.
    J Exp Med. 1966 Dec 1;124(6):1123-34 PMID: 5925318
  8. The regulation of pinocytosis in mouse macrophages. II. Factors inducing vesicle formation.
    J Exp Med. 1967 Feb 1;125(2):213-32 PMID: 4225263
  9. Kinetics of pinocytosis.
    Fed Proc. 1967 Jul-Aug;26(4):987-93 PMID: 6028782
  10. The ultrastructural basis of capillary permeability studied with peroxidase as a tracer.
    J Cell Biol. 1967 Oct;35(1):213-36 PMID: 6061717
  11. The uptake and digestion of iodinated human serum albumin by macrophages in vitro.
    J Exp Med. 1967 Nov 1;126(5):941-58 PMID: 6062005
  12. Uptake of protein by mammalian cells: an underdeveloped area. The penetration of foreign proteins into mammalian cells can be measured and their functions explored.
    Science. 1968 Jan 26;159(3813):390-6 PMID: 4169262
  13. Studies on blood capillaries. II. Transport of ferritin molecules across the wall of muscle capillaries.
    J Cell Biol. 1968 May;37(2):277-99 PMID: 5656395
  14. Adipose tissue dynamics and regulation, revisited.
    Ergeb Physiol. 1968;60:57-140 PMID: 4298672
  15. Vesicle fusion and formation at the surface of pinocytic vacuoles in macrophages.
    J Cell Biol. 1968 Sep;38(3):629-32 PMID: 5664229
  16. [Preparation for electron microscopy of adipose tissue cells, isolated as well as in situ].
    Z Gesamte Exp Med. 1968;148(4):255-74 PMID: 5708948
  17. Binding of long-chain fatty acids to bovine serum albumin.
    J Lipid Res. 1969 Jan;10(1):56-67 PMID: 5773785
  18. Alpha-aminoisobutyric acid uptake in isolated mouse fat cells.
    Biochim Biophys Acta. 1969 Jan 28;173(1):128-40 PMID: 4304768
  19. Structure-function relationships in the adipose cell. I. Ultrastructure of the isolated adipose cell.
    J Cell Biol. 1970 Aug;46(2):326-41 PMID: 5449178
  20. Structure-function relationships in the adipose cell. 3. Effects of bovine serum albumin on the metabolism of glucose and the release of nonesterified fatty acids and glycerol by the isolated adipose cell.
    J Cell Biol. 1970 Aug;46(2):354-61 PMID: 5449180
  21. Metabolism of isolated fat cells. VI. The effects of insulin, lipolytic hormones, and theophylline on glucose transport and metabolism in "ghosts".
    J Biol Chem. 1967 Dec 25;242(24):5751-6 PMID: 4319671
  22. Metabolic and ultrastructural changes induced in adipose tissue by insulin.
    J Biophys Biochem Cytol. 1960 Sep;8:83-101 PMID: 13687312
  23. Studies on the metabolism of adipose tissue. XII. The effects of insulin and epinephrine on free fatty acid and glycerol production in the presence and absence of glucose.
    Biochemistry. 1963 Mar-Apr;2:383-8 PMID: 13958062
  24. Electron microscopic study of adipose tissue (fat organs) with special reference to the transport of lipids between blood and fat cells.
    Z Zellforsch Mikrosk Anat. 1963;59:326-57 PMID: 13999052
  25. THE DIFFERENTIATION OF WHITE ADIPOSE CELLS. AN ELECTRON MICROSCOPE STUDY.
    J Cell Biol. 1963 Sep;18:663-79 PMID: 14064115
  26. ADIPOSE TISSUE. MORPHOLOGICAL CHANGES ASSOCIATED WITH LIPID MOBILIZATION.
    J Cell Biol. 1964 Jan;20:57-74 PMID: 14105216
  27. STUDIES ON THE METABOLISM OF ADIPOSE TISSUE. XVII. IN VITRO EFFECTS OF INSULIN UPON THE METABOLISM OF THE CARBOHYDRATE AND TRIGLYCERIDE STORES OF ADIPOSE TISSUE FROM FASTED-REFED RATS.
    Biochemistry. 1964 Nov;3:1696-702 PMID: 14235333
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.46.2.342
Published
1970-08-00
Pages
342-53
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2108022
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.46.2.342 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2