Binding and translation of turnip-yellow-mosaic-virus ribonucleic acid by skeletal-muscle ribosomes from normal and diabetic rats.

The Biochemical journal ·Vol. 117 ·No. 5 ·1970-05-00 ·Pages 899-905

Rolleston FS, Wool IG, Martin TE

Abstract

Ribosomes from skeletal muscle of diabetic rats were less active than normal ribosomes in protein synthesis directed by turnip-yellow-mosaic-virus RNA. The proportion of ribosomes from muscle of diabetic rats capable of binding turnip-yellow-mosaic-virus RNA was greater than normal, but there was no difference in the equilibrium constants for the binding reaction. The turnip-yellow-mosaic-virus RNA was bound preferentially to the small (40S) ribosomal subunit, whereas the decrease due to diabetes in its translation was associated with the large (60S) subunit. Thus the diminished capacity of ribosomes from muscle of diabetic rats to translate turnip-yellow-mosaic-virus RNA was not the result of decreased binding of the template.

MeSH Terms

Animals Binding Sites Carbon Isotopes Diabetes Mellitus, Experimental/metabolism Genetic Code In Vitro Techniques Muscles/metabolism Protein Biosynthesis RNA, Viral/metabolism Rats Ribosomes/metabolism

Chemicals

Carbon Isotopes RNA, Viral

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Rolleston F S

Wool I G

Martin T E

References (12)

12 references, click to expand

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The ribosome-binding sites in turnip yellow mosaic virus RNA.
Proc Natl Acad Sci U S A. 1966 May;55(5):1269-76 PMID: 5334204
Insulin regulation of protein synthesis by muscle ribosomes: effect of the hormone on translation of messenger RNA for a regulatory protein.
Proc Natl Acad Sci U S A. 1966 Sep;56(3):991-8 PMID: 4165816
The relation of RNA and protein synthesis to the sedimentation of muscle ribosomes: effect of diabetes and insulin.
Proc Natl Acad Sci U S A. 1967 Jun;57(6):1885-92 PMID: 4166533
On the significance of ribosome dimers in extracts of animal cells.
J Mol Biol. 1967 Sep 14;28(2):211-23 PMID: 6052638
Determination of the number of active muscle ribosomes: effect of diabetes and insulin.
Proc Natl Acad Sci U S A. 1967 Dec;58(6):2401-7 PMID: 5242216
Mode of action of insulin in the regulation of protein biosynthesis in muscle.
Recent Prog Horm Res. 1968;24:139-213 PMID: 4302428
Formation of active hybrids from subunits of muscle ribosomes from normal and diabetic rats.
Proc Natl Acad Sci U S A. 1968 Jun;60(2):569-74 PMID: 5248814
The precision of ultraviolet absorption measurements in the Schmidt-Thannhauser procedure for nucleic acid estimation.
Biochim Biophys Acta. 1962 May 14;55:571-83 PMID: 13893456
Cell-free protein synthesis: the association of viral RNA and E. coli ribosomes.
Proc Natl Acad Sci U S A. 1963 Apr;49:511-7 PMID: 13960835
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J Mol Biol. 1963 Oct;7:323-33 PMID: 14066611
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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1170899

Published

1970-05-00

Pages

899-905

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1179048

Subset

External Links

PubMed source DOI: 10.1042/bj1170899 PMC Full Text View journal details

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Binding and translation of turnip-yellow-mosaic-virus ribonucleic acid by skeletal-muscle ribosomes from normal and diabetic rats.

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