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PMID: 5473896 Published · ppublish English Journal Article

Ribonucleic acid synthesis during microcyst formation in Myxococcus xanthus: characterization by deoxyribonucleic acid-ribonucleic acid hybridization.

Journal of bacteriology ·Vol. 104 ·No. 1 ·1970-10-00 ·Pages 275-82

Okano P, Bacon K, Rosenberg E

Abstract

The technique of deoxyribonucleic acid-ribonucleic acid (RNA) hybridization was used to compare the RNA synthesized during vegetative growth and microcyst formation in Myxococcus xanthus. All classes of RNA, including ribosomal RNA, were synthesized during microcyst formation. The results indicate that the ribosomal RNA synthesized during microcyst formation was indistinguishable from that made during vegetative growth. Hybridization competition experiments demonstrated that certain messenger RNA species are synthesized only during vegetative growth, whereas others are synthesized only during microcyst formation. The synthesis of a new species of RNA polymerase does not appear to be responsible for differential transcription during morphogenesis in M. xanthus since the rifampicin sensitivity of transcription was conserved during microcyst formation.

MeSH Terms
Bacteria/drug effects,enzymology,metabolism DNA, Bacterial/biosynthesis,metabolism Hybridization, Genetic Molecular Biology Mutation Nucleosides/metabolism RNA Nucleotidyltransferases/metabolism RNA, Bacterial/biosynthesis Rifampin/pharmacology Tritium
Chemicals
DNA, Bacterial Nucleosides RNA, Bacterial Tritium RNA Nucleotidyltransferases Rifampin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Okano P
Bacon K
Rosenberg E
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41 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.104.1.275-282.1970
Published
1970-10-00
Pages
275-82
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC248211
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.104.1.275-282.1970 PMC Full Text View journal details
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