Restricted replication of frog virus 3 in selected variants of BHK cells.

Journal of virology ·Vol. 6 ·No. 3 ·1970-09-00 ·Pages 303-9

Vilaginès R, McAuslan BR

Abstract

Synthesis and maturation of frog virus 3 deoxyribonucleic acid (DNA) in BHK cells and selected variants, in chick fibroblasts, and in minnow cells were compared. Wide ranges in rates of DNA synthesis and assembly of virions were found. At least three variants of BHK cells can be obtained: (i) fully permissive, characterized by rapid DNA synthesis and assembly to give a high yield of infective virus; (ii) semipermissive, in which viral DNA is synthesized slowly for extended periods of time, and the yield of infective virus is poor; and (iii) nonpermissive, in which virus adsorbs to cells and arrests host functions but viral DNA is not synthesized. Kinetics of appearance of virions and procedures for their separation from cell extracts are described.

MeSH Terms

Animals Anura Cell Line Centrifugation, Density Gradient Chick Embryo Cricetinae Culture Techniques DNA Viruses/metabolism DNA, Viral/biosynthesis Deoxyribonucleases Fibroblasts Fishes Kidney Thymidine/metabolism Time Factors Tritium Virus Replication

Chemicals

DNA, Viral Tritium Deoxyribonucleases Thymidine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Vilaginès R

McAuslan B R

References (10)

10 references, click to expand

Viruses and renal carcinoma of Rana pipiens. I. The isolation and properties of virus from normal and tumor tissue.
Virology. 1966 May;29(1):133-48 PMID: 5936626
The isolation and properties of viruses from Rana pipiens: their possible relationship to the renal adenocarcinoma of the leopard frog.
Ann N Y Acad Sci. 1965 Aug 10;126(1):237-55 PMID: 5220161
A permanent cell line from the fathead minnow (Pimephales promelas).
Ann N Y Acad Sci. 1965 Aug 10;126(1):555-65 PMID: 5220177
RNA metabolism in the HeLa cell nucleus.
J Mol Biol. 1966 May;17(1):117-30 PMID: 5961144
Some biophysical properties of frog viruses and their DNA.
Proc Natl Acad Sci U S A. 1966 Oct;56(4):1155-7 PMID: 4961525
Viruses and renal carcinoma of Rana pipiens. 3. The relationship between input multiplicity of infection and inclusion body formation in frog virus 3-infected cells.
Virology. 1967 Sep;33(1):137-44 PMID: 4166975
Viruses and renal carcinoma of Rana pipiens. VI. Interrelationships of macromolecular synthesis and infectious virus production in frog virus 3-infected BHK 21/13 cells.
Virology. 1968 Feb;34(2):240-9 PMID: 5643637
Deoxyribonucleic acid synthesis in FV-3-infected mammalian cells.
J Virol. 1968 Oct;2(10):1006-15 PMID: 5749374
Biophysical properties of frog virus and its deoxyribonucleic acid: fate of radioactive virus in the early stage of infection.
J Virol. 1969 Oct;4(4):339-47 PMID: 4980848
SYRIAN HAMSTER FIBROBLAST CELL LINE BHK21 AND ITS DERIVATIVES.
Nature. 1964 Sep 26;203:1355-7 PMID: 14207308

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.6.3.303-309.1970

Published

1970-09-00

Pages

303-9

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC376123

Subset

External Links

PubMed source DOI: 10.1128/JVI.6.3.303-309.1970 PMC Full Text View journal details

Analysis Services

Restricted replication of frog virus 3 in selected variants of BHK cells.

Contact

WeChat Official Account