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PMID: 5495151 Published · ppublish English Journal Article

Chemical properties of guinea-pig immunoglobulins gamma-1 G, gamma-2 G and gamma M.

The Biochemical journal ·Vol. 120 ·No. 4 ·1970-12-00 ·Pages 787-95

Leslie RG, Cohen S

Abstract

The isolation of guinea-pig immunoglobulins gamma(1)G, gamma(2)G and gammaM are described and methods for separating the polypeptide chains of each examined. The molecular weights, extinction coefficients and carbohydrate and amino acid compositions of the immunoglobulins and their constituent chains have been analysed. The findings provide a basis for further studies attempting to relate structural differences to distinct biological properties of guinea-pig immunoglobulins.

MeSH Terms
Amino Acids/analysis Animals Chemical Precipitation Chromatography, DEAE-Cellulose Chromatography, Gel Cysteine Fucose/analysis Guinea Pigs Hexosamines/analysis Hexoses/analysis Immunoelectrophoresis Immunoglobulin G/analysis Immunoglobulin M/analysis,isolation & purification Immunoglobulins/analysis,isolation & purification Iodoacetates Mercaptoethanol Molecular Weight Oxidation-Reduction Peptides/analysis Quaternary Ammonium Compounds Rabbits Ultracentrifugation
Chemicals
Amino Acids Hexosamines Hexoses Immunoglobulin G Immunoglobulin M Immunoglobulins Iodoacetates Peptides Quaternary Ammonium Compounds Fucose Mercaptoethanol Cysteine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Leslie R G
Cohen S
References (32)
32 references, click to expand
  1. Properties of guinea pig 7S antibodies. III. Identification of antibodies involved in complement fixation and hemolysis.
    J Exp Med. 1963 Jun 1;117:965-81 PMID: 13971679
  2. Properties of guinea pig 7S antibodies. II. Identification of antibodies involved in passive cutaneous and systemic anaphylaxis.
    J Exp Med. 1963 Jun 1;117:951-64 PMID: 13940786
  3. Non-complement-fixing IgM antibodies in the guinea pig.
    J Immunol. 1969 Sep;103(3):423-8 PMID: 5805428
  4. Opsonic, cytophilic and agglutinating activity of guinea-pig gamma-2 and gamma-M anti-Salmonella antibodies.
    Immunology. 1969 Mar;16(3):361-71 PMID: 4890312
  5. Immunoelectrophoretic identification of guinea pig anti-insulin antibodies.
    J Immunol. 1962 Nov;89:736-44 PMID: 14002202
  6. Polypeptide chain structure of rabbit immunoglobulins. I. gamma-G-immunoglobulin.
    Biochemistry. 1966 Jan;5(1):259-67 PMID: 4161041
  7. AVIDIN. 2. PURIFICATION AND COMPOSITION.
    Biochem J. 1963 Dec;89:591-9 PMID: 14101980
  8. Polypeptide chain structure of rabbit immunoglobulins. 3. Secretory gamma-A-immunoglobulin from colostrum.
    Biochemistry. 1967 Feb;6(2):503-12 PMID: 4167322
  9. Properties of guinea pig 7S antibodies. I. Electrophoretic separation of two types of guinea pig 7S antibodies.
    J Exp Med. 1963 Jun 1;117:937-49 PMID: 13967257
  10. DISSOCIATION OF RABBIT GAMMA-GLOBULIN INTO HALF-MOLECULES AFTER REDUCTION OF ONE LABILE DISULFIDE BOND.
    Biochemistry. 1964 Jun;3:863-9 PMID: 14211630
  11. The fixation of guinea pig complement by gamma 1 and gamma 2 immunoglobulins.
    J Immunol. 1969 Jan;102(1):269-71 PMID: 4180091
  12. Polypeptide chain structure of rabbit immunoglobulins. II. gamma-M-immunoglobulin.
    Biochemistry. 1966 Jan;5(1):267-76 PMID: 4161042
  13. Properties of antibodies cytophilic for macrophages.
    J Exp Med. 1966 Jan 1;123(1):119-44 PMID: 4159249
  14. PROPERTIES OF GUINEA PIG 7S ANTIBODIES. IV. ANTIBODY RESPONSE TO E. COLI BACTERIA.
    Proc Soc Exp Biol Med. 1963 Oct;114:52-6 PMID: 14076911
  15. The production of skin-sensitizing antibody in the guinea-pig.
    Int Arch Allergy Appl Immunol. 1963;22:156-65 PMID: 14000351
  16. HEXOSE CONTENT OF GUINEA PIG GAMMA-1 AND GAMMA-2 IMMUNOGLOBULINS.
    Proc Soc Exp Biol Med. 1965 Feb;118:336-42 PMID: 14268615
  17. AMINO ACID COMPOSITIONS OF HUMAN AND RABBIT GAMMA-GLOBULINS AND OF THE FRAGMENTS PRODUCED BY REDUCTION.
    Biochem J. 1963 Aug;88:228-34 PMID: 14063860
  18. Production of 7 S immunoglobulins in immunized guinea pigs.
    J Immunol. 1966 Aug;97(2):159-64 PMID: 4162284
  19. Skin-sensitizing properties of guinea pig antibodies to keyhole limpet hemocyanin.
    J Immunol. 1968 Jun;100(6):1245-54 PMID: 4972186
  20. Presence of two types of L polypeptide chains in guinea pig 7S immunoglobulins.
    J Exp Med. 1966 Nov 1;124(5):787-803 PMID: 4959010
  21. Chemical structure of light chains.
    Proc R Soc Lond B Biol Sci. 1966 Nov 22;166(1003):138-49 PMID: 4382708
  22. [A micro-method of immuno-electrophoresis].
    Int Arch Allergy Appl Immunol. 1955;7(2):103-10 PMID: 13262900
  23. Purification of antibodies with immunoadsorbents prepared using bromoacetyl cellulose.
    Immunochemistry. 1967 Jan;4(1):11-22 PMID: 6030410
  24. Hypersensitivity to Ascaris antigens. II. The skin-sensitizing properties of 7 S-gamma-2 antibody from sensitized guinea pigs as tested in guinea pigs.
    J Immunol. 1967 Aug;99(2):347-56 PMID: 4226614
  25. Carbohydrates in protein. 2. The hexose, hexosamine, acetyl and amide-nitrogen content of hen's-egg albumin.
    Biochem J. 1960 Nov;77:239-47 PMID: 13790184
  26. THE ARRANGEMENT OF THE PEPTIDE CHAINS IN GAMMA-GLOBULIN.
    Biochem J. 1963 Aug;88:220-8 PMID: 14063859
  27. Evidence for C-terminal intra-subunit disulphide bridges between immunoglobulin-M heavy chains.
    FEBS Lett. 1970 Apr 2;7(2):175-176 PMID: 11947461
  28. The disulphide bonds of the heavy chain of rabbit immunoglobulin G.
    Biochem J. 1970 Jan;116(2):261-8 PMID: 5461107
  29. Further comparison of guinea pig gamma-1-and gamma-2-immunoglobulins. Amino acid compositions of the polypeptide chains and papain fragments.
    Immunochemistry. 1969 Mar;6(2):235-40 PMID: 5778778
  30. CHARACTERIZATION OF A HUMAN MACROGLOBULIN. I. THE MOLECULAR WEIGHT OF ITS SUBUNIT.
    J Biol Chem. 1965 Aug;240:3325-33 PMID: 14328368
  31. Cyanogen bromide cleavage and partial sequence of the heavy chain of a pathological immunoglobulin G.
    Biochem J. 1967 Aug;104(2):616-26 PMID: 4167412
  32. Methods for the quantitative estimation of N-acetylneuraminic acid and their application to hydrolysates of sialomucoids.
    Biochem J. 1961 Nov;81:384-92 PMID: 13860975
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1200787
Published
1970-12-00
Pages
787-95
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1179672
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1200787 PMC Full Text View journal details
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