Ribonucleic acid transcriptase acitvity in purified wound tumor virus.

Journal of virology ·Vol. 6 ·No. 2 ·1970-08-00 ·Pages 194-8

Black DR, Knight CA

Abstract

Purified wound tumor virus was found to possess an associated ribonucleic acid (RNA) transcriptase. The product of transcriptase synthesis was shown to be single-stranded RNA which annealed specifically to wound tumor viral RNA.

MeSH Terms

Centrifugation, Density Gradient Chemical Precipitation Dactinomycin Genetic Code Genetics, Microbial Guanine Nucleotides/metabolism Hydrogen-Ion Concentration Indicators and Reagents Mercaptoethanol Plant Viruses/enzymology,growth & development,isolation & purification RNA Nucleotidyltransferases/metabolism RNA, Viral/isolation & purification Spectrophotometry Sucrose Temperature Tobacco Mosaic Virus Trichloroacetic Acid Tritium Uracil Nucleotides/metabolism Virus Replication

Chemicals

Guanine Nucleotides Indicators and Reagents RNA, Viral Uracil Nucleotides Tritium Dactinomycin Sucrose Trichloroacetic Acid Mercaptoethanol RNA Nucleotidyltransferases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Black D R

Knight C A

References (8)

8 references, click to expand

Chromatography of nucleic acids on hydroxyapatite. 3. Chromatography of RNA and polyribonucleotides.
Biochim Biophys Acta. 1969 Feb 18;174(2):449-57 PMID: 5813316
RNA polymerase activity in purified reoviruses.
Proc Natl Acad Sci U S A. 1968 Dec;61(4):1462-9 PMID: 4179180
Characterization of a Ribonucleic Acid Polymerase Activity Associated with Purified Cytoplasmic Polyhedrosis Virus of the Silkworm Bombyx mori.
J Virol. 1969 Dec;4(6):857-65 PMID: 16789118
Reovirus: RNA polymerase activity in purified virions.
Biochem Biophys Res Commun. 1968 Dec 30;33(6):895-901 PMID: 5750853
Plant virus-induced RNA polymerase: properties of the enzyme partly purified from cucumber cotyledons infected with cucumber mosaic virus.
Virology. 1969 Sep;39(1):54-65 PMID: 5807971
Studies on the in vitro transcription of reovirus RNA catalyzed by reovirus cores.
Virology. 1969 Dec;39(4):822-31 PMID: 5358831
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Comparison of the ribonucleic Acid subunits of reovirus, cytoplasmic polyhedrosis virus, and wound tumor virus.
J Virol. 1969 Dec;4(6):851-6 PMID: 16789117

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.6.2.194-198.1970

Published

1970-08-00

Pages

194-8

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC376108

Subset

External Links

PubMed source DOI: 10.1128/JVI.6.2.194-198.1970 PMC Full Text View journal details

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Ribonucleic acid transcriptase acitvity in purified wound tumor virus.

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