Chloride-dependent restoration of coupling by oligomycin in rat liver mitochondria.

The Biochemical journal ·Vol. 152 ·No. 1 ·1975-10-00 ·Pages 115-9

Ariel N, Avi-Dor Y

Abstract

In rat liver mitochondria suspended in KC1 medium, oligomycin interfered with the effect of uncouplers on energy conservation. It antagonized the effect of uncouplers that are weak acids (2,4-dinitrophenol etc.), but enhanced that of the lipid-penetrating cation NN-dimethyl-N'N'-dibenzylammonium. Oligomycin caused none of the above effects when Br- or NO-/3 was substituted for C1- as the major anionic species in the assay medium. The concentration of oligomycin that exerted the above-mentioned effects was lower than that necessary for the inhibition of energy transfer, but was in the range that induced C1- permeation through the cristae membrane. The possible connexion between the effect of oligomycin on C1- permeation and its interference with the action of uncouplers is discussed.

MeSH Terms

Adenosine Diphosphate/pharmacology Adenosine Triphosphatases/analysis Animals Bromides/metabolism Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone/antagonists & inhibitors Chlorides/metabolism Dicumarol/antagonists & inhibitors Dinitrophenols/antagonists & inhibitors,pharmacology Mitochondria, Liver/drug effects,metabolism Mitochondrial Swelling/drug effects Nitrates/metabolism Oligomycins/pharmacology Oxidative Phosphorylation/drug effects Oxygen Consumption/drug effects Quaternary Ammonium Compounds/antagonists & inhibitors Rats Uncoupling Agents/antagonists & inhibitors

Chemicals

Bromides Chlorides Dinitrophenols Nitrates Oligomycins Quaternary Ammonium Compounds Uncoupling Agents Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone Adenosine Diphosphate Dicumarol Adenosine Triphosphatases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Ariel N

Avi-Dor Y

References (10)

10 references, click to expand

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Chloride-dependent uncoupling mediated by oligomycin in rat liver mitochondria.
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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1520115

Published

1975-10-00

Pages

115-9

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1172446

Subset

External Links

PubMed source DOI: 10.1042/bj1520115 PMC Full Text View journal details

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Chloride-dependent restoration of coupling by oligomycin in rat liver mitochondria.

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