The in vitro transformation of frozen-stored lymphocytes in the mixed lymphocyte reaction and in culture with phytohemagglutinin and specific antigens.

The Journal of experimental medicine ·Vol. 132 ·No. 3 ·1970-09-01 ·Pages 401-16

Mangi RJ, Mardiney MR

Abstract

We have investigated the in vitro transformation of lymphocytes that have been frozen and stored at low temperatures. Cells frozen at different times and stored for various times do transform in a reproducible manner when placed in culture with PHA or as one population of the two-way or the one-way MLR. When frozen-stored lymphocytes are cultured with specific antigen or as both partners in the MLR the response is minimal. The freezing process destroys neutrophils, and the remaining population transforms in a manner similar to cultures of purified lymphocytes. Practical aspects of the technique are discussed and we have suggested possible practical applications for future investigation.

MeSH Terms

Antigen-Antibody Reactions Antigens Cells, Cultured Freezing Humans Lectins Lymphocyte Activation

Chemicals

Antigens Lectins

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Mangi R J

Mardiney M R

References (30)

30 references, click to expand

The effect of skin homograft rejection on recipient and donor mixed leukocyte cultures.
J Exp Med. 1965 Oct 1;122(4):651-64 PMID: 5321601
MITOGENIC STIMULATION OF PERIPHERAL LYMPHOCYTE CULTURES BY AUTOLOGOUS LYMPHOCYTE EXTRACTS IN AUTOIMMUNE DISEASES.
Lancet. 1963 Nov 16;2(7316):1030-1 PMID: 14062722
Lymphocyte transformation in autoimmune disorders: a preliminary study.
J Pediatr. 1966 Dec;69(6):1129-31 PMID: 5953840
Impaired in vitro lymphocyte transformation in patients with ataxia-telangiectasis.
Br Med J. 1966 Aug 6;2(5509):330-3 PMID: 5920654
Phytohaemagglutinin and lymphocytes from acute lymphocytic leukaemia.
Lancet. 1966 Jun 4;1(7449):1265-6 PMID: 4161226
One-way stimulation in mixed leukocyte cultures.
Science. 1966 Jul 29;153(3735):545-7 PMID: 5938778
PHAGOCYTIC ACTIVITY AND ANTIGENIC INTEGRITY OF LEUKOCYTES PRESERVED WITH DIMETHYL SULFOXIDE AT A CRYOGENIC TEMPERATURE (--196 DEGREES C).
Vox Sang. 1965 May-Jun;10:382-4 PMID: 14332953
Impaired phytohaemagglutinin-induced cytotoxicity in vitro of lymphocytes from patients with Hodgkin's disease or chronic lymphatic leukaemia.
Clin Exp Immunol. 1967 May;2(3):351-60 PMID: 6035198
Lymphocyte transformation in thymectomized and nonthymectomized patients with myasthenia gravis.
Br Med J. 1967 Jun 10;2(5553):679-81 PMID: 6024528
Quantitative assay of antigenic disparity at hl-a-the major histocompatibility locus in man.
J Exp Med. 1968 Oct 1;128(4):639-51 PMID: 19867302
Detection of leukoagglutinins with dimethylsulfoxide (DMSO) protected leukocytes frozen with liquid nitrogen.
Vox Sang. 1965 Sep-Oct;10(5):543-51 PMID: 5864814
LEUCOCYTE CULTURES WITH PHYTOHAEMAGGLUTININ IN CHRONIC LYMPHATIC LEUKAEMIA.
Lancet. 1964 Sep 12;2(7359):563-5 PMID: 14172353
THE RELATIONSHIP OF IN VITRO LYMPHOCYTE COMPATIBILITY TO HOMOGRAFT SENSITIVITY IN MAN.
Ann N Y Acad Sci. 1964 Nov 30;120:303-6 PMID: 14233879
Lymphocyte number and response to phytohae- magglutinin in chronic lymphocytic leukaemia.
Scand J Haematol. 1966;3(5):375-82 PMID: 5958145
Preservation of human normal and leukemic cells with dimethyl sulfoxide at -80 degrees C.
Cryobiology. 1967 May-Jun;3(6):445-55 PMID: 6058185
Effects of phytohemagglutinin on rat and normal and leukemic human blood cells.
Proc Soc Exp Biol Med. 1963 May;113:191-4 PMID: 13987041
Lymphocytes from leukaemic blood cultured with phytohemagglutinin.
Eur J Cancer. 1965 Nov;1(3):259-64 PMID: 5874722
Factors affecting the reactivity of human lymphocytes in vitro. I. Cell number, duration of culture and surface area.
J Immunol. 1967 Aug;99(2):413-9 PMID: 6031208
The transformation of column-purified lymphocytes with nonspecific and specific antigenic stimuli.
J Immunol. 1968 Aug;101(2):262-7 PMID: 5668026
In vitro lymphocyte transformation in ataxia telangiectasia.
J Pediatr. 1966 Mar;68(3):477-9 PMID: 5903320
IMMUNOLOGIC AND CYTOGENETIC STUDIES OF CHRONIC LYMPHOCYTIC LEUKEMIC CELLS.
Blood. 1965 Aug;26:121-32 PMID: 14332475
Heterogeneity of lymphocytes in chronic lymphocytic leukaemia.
Lancet. 1966 Jul 23;2(7456):200-2 PMID: 4161167
An assessment of the mixed leucocyte reaction in renal failure.
Lancet. 1966 Mar 26;1(7439):682-5 PMID: 4159600
Quantitative studies on the mixed lymphocyte interaction in rats. II. Relationship of the proliferative response to the immunologic status of the donors.
J Exp Med. 1967 Oct 1;126(4):655-65 PMID: 6055760
DNA AND RNA SYNTHESIS OF CIRCULATING ATYPICAL LYMPHOCYTES IN INFECTIOUS MONONUCLEOSIS.
Blood. 1965 Feb;25:197-203 PMID: 14267695
Studies of the delayed response of phytohemagglutinin (PHA) stimulated lymphocytes in 25 chronic lymphatic leukemia patients before and during therapy.
Blood. 1969 Aug;34(2):166-78 PMID: 5794114
Impaired in vitro lymphocyte transformation in Hodgkin's disease.
N Engl J Med. 1965 Nov 4;273(19):1006-12 PMID: 5844228
EFFECTS OF PHYTOHAEMAGGLUTININ ON BLOOD-CULTURES OF CHRONIC LYMPHOCYTIC LEUKAEMIAS.
Lancet. 1964 Mar 21;1(7334):667-8 PMID: 14107951
Stimulation of lymphocytes by autologous leukaemic cells in acute leukaemia.
Nature. 1969 Oct 18;224(5216):277-9 PMID: 5259376
Chemotherapy in Burkitt's Tumour.
Lancet. 1966 Oct 15;2(7468):856 PMID: 4162182

Article Info

Journal

The Journal of experimental medicine

Abbr.

J Exp Med

ISSN

0022-1007

DOI

10.1084/jem.132.3.401

Published

1970-09-01

Pages

401-16

Language

English

Region

United States

NLM ID

2985109R

PMCID

PMC2138801

Subset

External Links

PubMed source DOI: 10.1084/jem.132.3.401 PMC Full Text View journal details

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The in vitro transformation of frozen-stored lymphocytes in the mixed lymphocyte reaction and in culture with phytohemagglutinin and specific antigens.

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