Purification and crystallization of avidin.

The Biochemical journal ·Vol. 118 ·No. 1 ·1970-06-00 ·Pages 67-70

Green NM, Toms EJ

Abstract

An improved purification, including crystallization, of avidin from hen's-egg white is described. The product bound 15.1mug of biotin/mg, corresponding to an equivalent weight of 16200. The amino acid composition showed an integral number of amino acid residues per 16200g, which suggested that each molecule of avidin (mol. wt. approx. 66000) contained four identical subunits.

MeSH Terms

Amino Acids/analysis Animals Biotin Chickens Chromatography Crystallization Molecular Weight Ovalbumin/analysis,isolation & purification Protein Binding

Chemicals

Amino Acids Biotin Ovalbumin

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Green N M

Toms E J

References (7)

7 references, click to expand

Evidence for a genetic relationship between avidins and lysozymes.
Nature. 1968 Jan 20;217(5125):254-6 PMID: 5639130
The effect of N-bromosuccinimide on the sub-unit structure of acidin and its complexes with biotin.
Biochem J. 1968 Nov;110(1):59-66 PMID: 5722693
A preliminary crystallographic investigation of avidin.
Biochem J. 1970 Jun;118(1):71-2 PMID: 5472157
[Malignant tumors in otorhinolaryngology. II. Tumors of the nose and its sinuses. 2. Treatment methods, results, problems of after care and rehabilitation].
Arch Geschwulstforsch. 1970;35(2):129-45 PMID: 4331034
Avidin. I. Isolation and characterization of the protein and nucleic acid.
Arch Biochem Biophys. 1952 Jul;39(1):80-96 PMID: 12997130
AMINO ACID COMPOSITIONS OF HUMAN AND RABBIT GAMMA-GLOBULINS AND OF THE FRAGMENTS PRODUCED BY REDUCTION.
Biochem J. 1963 Aug;88:228-34 PMID: 14063860
AVIDIN. 2. PURIFICATION AND COMPOSITION.
Biochem J. 1963 Dec;89:591-9 PMID: 14101980

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1180067

Published

1970-06-00

Pages

67-70

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1179080

Subset

External Links

PubMed source DOI: 10.1042/bj1180067 PMC Full Text View journal details

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Purification and crystallization of avidin.

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