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PMID: 5529847 Published · ppublish English Journal Article

Transcription in vitro by reovirus-associated ribonucleic acid-dependent polymerase.

Journal of virology ·Vol. 6 ·No. 1 ·1970-07-00 ·Pages 1-11

Banerjee AK, Shatkin AJ

Abstract

Digestion of purified reovirus type 3 with chymotrypsin degrades 70% of the viral protein and converts the virions to subviral particles (SVP). The SVP contain 3 of the 6 viral structural proteins and all 10 double-stranded ribonucleic acid (RNA) genome segments but not adenine-rich, single-stranded RNA. An RNA polymerase which is structurally associated with SVP transcribes one strand of each genome segment by a conservative mechanism in vitro. The single-stranded products include large (1.2 x 10(6) daltons), medium (0.7 x 10(6) daltons), and small (0.4 x 10(6) daltons) molecules which hybridize exclusively with the corresponding genome segments. The enzyme obtained by heating virions at 60 C synthesizes similar products. Kinetic and pulse-chase studies indicate that the different-sized products are synthesized simultaneously but at rates which are in the order: small > medium > large.

MeSH Terms
Acrylates Animals Centrifugation, Density Gradient Cesium Chlorides Chymotrypsin/metabolism Electrophoresis Gels Genetic Code Genetics, Microbial Hot Temperature Hybridization, Genetic L Cells Mice Molecular Weight Nucleotides Paper Phosphates Phosphorus Isotopes RNA Nucleotidyltransferases/metabolism RNA, Messenger/biosynthesis RNA, Viral/analysis,biosynthesis Reoviridae/enzymology,metabolism Tritium
Chemicals
Acrylates Chlorides Gels Nucleotides Phosphates Phosphorus Isotopes RNA, Messenger RNA, Viral Tritium Cesium RNA Nucleotidyltransferases Chymotrypsin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Banerjee A K
Shatkin A J
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38 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.6.1.1-11.1970
Published
1970-07-00
Pages
1-11
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC376083
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.6.1.1-11.1970 PMC Full Text View journal details
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