Home LiteratureArticle Details
PMID: 55335 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

The fluorescence localization of mouse satellite DNA in interphase nuclei.

Chromosoma ·Vol. 54 ·No. 1 ·1976-01-27 ·Pages 27-31

Mayfield JE, Ellison JR

Abstract

Nuclei from various mouse tissues exhibit a pattern of fluorescence characteristic of the cell type when stained with the fluorescent compound Hoechst 33258. When such preparations are hybridized in situ with 3H-RNA complementary to the A-T rich satellite of mouse, it is clearly seen that only the fluorescent regions of the nuclei contain the satellite DNA. Thus Hoechst 33258 allows the precise localization of satellite DNA at all stages of the mouse cell cycle.

MeSH Terms
Animals Benzimidazoles Cell Division Chromatin/analysis DNA/analysis DNA, Satellite/analysis Fluorescent Dyes Male Mice Nucleic Acid Hybridization Staining and Labeling
Chemicals
Benzimidazoles Chromatin DNA, Satellite Fluorescent Dyes DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mayfield J E
Ellison J R
References (8)
8 references, click to expand
  1. Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms.
    Science. 1968 Aug 9;161(3841):529-40 PMID: 4874239
  2. Chromosomal localization of mouse satellite DNA.
    Science. 1970 Jun 12;168(3937):1356-8 PMID: 5462793
  3. Optical studies of the interaction of 33258 Hoechst with DNA, chromatin, and metaphase chromosomes.
    Chromosoma. 1975 Nov 11;52(4):297-316 PMID: 1192901
  4. Fluorometric properties of the bibenzimidazole derivative Hoechst 33258, a fluorescent probe specific for AT concentration in chromosomal DNA.
    Chromosoma. 1974;46(3):255-60 PMID: 4136742
  5. Isolation and characterization of mouse and guinea pig satellite deoxyribonucleic acids.
    Biochemistry. 1968 Dec;7(12):4373-9 PMID: 5700661
  6. Chromosomal and nuclear location of mouse satellite DNA in individual cells.
    Nature. 1970 Mar 7;225(5236):912-5 PMID: 5415126
  7. Staining of constitutive heterochromatin in mammalian chromosomes with a new fluorochrome.
    Exp Cell Res. 1972 Nov;75(1):122-6 PMID: 4117919
  8. The organization of interphase chromatin in drosophilidae: the self adhesion of chromatin containing the same DNA sequences.
    Chromosoma. 1975 Sep 15;52(1):37-48 PMID: 1175457
Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/BF00331830
Published
1976-01-27
Pages
27-31
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00331830 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2