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PMID: 5534448 Published · ppublish English Journal Article

Cellular differentiation in the thymus. 3. Surface properties of rat thymus and lymph node cells separated on density gradients.

The Journal of experimental medicine ·Vol. 132 ·No. 6 ·1970-12-01 ·Pages 1107-21

Colley DG, Wu AY, Waksman BH

Abstract

Young adult rat thymus and lymph node cell subpopulations were obtained by differential flotation on discontinuous BSA density gradients and assayed for properties characteristic of mature thymus-derived lymphocytes. One such subpopulation (C) of thymocytes was enriched in its ability to respond mitotically to a hemiallogeneic MLR stimulus, to localize in the parenchyma of lymph nodes and spleen, and to initiate a GVH reaction in a suitable host. These cells did not respond well to mitotic stimulation by PHA, they were lighter in density than the majority of mature lymph node thymus-derived lymphocytes, and they possessed a thymus-specific antigen (RTA) not present on peripheral lymphoid cells. We conclude that the acquisition of peripheral properties occurs sequentially, during an intrathymic differentiation cycle or shortly after the cells leave the thymus.

MeSH Terms
Animals Cell Differentiation Centrifugation, Density Gradient Culture Media DNA/biosynthesis In Vitro Techniques Lectins Liver/cytology Lymph Nodes/cytology Lymphocyte Activation Lymphocytes/immunology Rabbits Rats Spleen/cytology Surface Properties Thymus Gland/cytology
Chemicals
Culture Media Lectins DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Colley D G
Wu A Y
Waksman B H
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40 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.132.6.1107
Published
1970-12-01
Pages
1107-21
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2180505
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.132.6.1107 PMC Full Text View journal details
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