Isolation and properties of a new species of ribonucleic acid synthesized in sporulating cells of Saccharomyces cerevisiae.

Journal of bacteriology ·Vol. 105 ·No. 3 ·1971-03-00 ·Pages 831-6

Kadowaki K, Halvorson HO

Abstract

A new species of ribonucleic acid (RNA) was detected in sporulating culture of Saccharomyces cerevisiae. This RNA was isolated by sucrose density gradient centrifugation and polyacrylamide gel electrophoresis and partially characterized. It has a sedimentation coefficient of approximately 20S and a nucleotide composition distinct from other known RNA species in yeast, and it hybridizes with nuclear but not with mitochondrial deoxyribonucleic acid.

MeSH Terms

Acrylates Autoanalysis Centrifugation, Density Gradient Cesium Chemical Precipitation Chlorides Culture Media DNA Electrophoresis Gels Genetics, Microbial Hybridization, Genetic Nucleotides/analysis Phenols Phosphorus Isotopes RNA/analysis,isolation & purification Saccharomyces/analysis,growth & development Spores/growth & development Sucrose

Chemicals

Acrylates Chlorides Culture Media Gels Nucleotides Phenols Phosphorus Isotopes Cesium Sucrose RNA DNA

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Kadowaki K

Halvorson H O

References (16)

16 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.105.3.831-836.1971

Published

1971-03-00

Pages

831-6

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC248507

Subset

External Links

PubMed source DOI: 10.1128/jb.105.3.831-836.1971 PMC Full Text View journal details

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Isolation and properties of a new species of ribonucleic acid synthesized in sporulating cells of Saccharomyces cerevisiae.

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