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PMID: 5547996 Published · ppublish English Journal Article

Mechanism of conversion of the salmonella O antigen by bacteriophageepsilon 34.

Journal of bacteriology ·Vol. 105 ·No. 3 ·1971-03-00 ·Pages 927-36

Wright A

Abstract

The structural determinants for antigen 34 in the E group salmonella are glucosyl substituents on the galactosyl units of the O antigen which has a mannosylrhamnosylgalactose repeating sequence. The temperate bacteriophage epsilon(34) brings about the production of antigen 34. It has been shown here that glucose is transferred from uridine diphosphate glucose to the O antigen via a glucosyl-lipid intermediate in a two-step reaction. Glucose is linked through carbon 1 to the lipid by a phosphodiester bridge, the glucosyl bond having the beta-anomeric configuration. The lipid is a C(55)-polyisoprenoid alcohol, each isoprene unit having one double bond. It is the same lipid which is involved in the synthesis of the O antigen repeating sequence.

MeSH Terms
Antigens/analysis Carbon Isotopes Chemical Phenomena Chemistry Chromatography, DEAE-Cellulose Chromatography, Paper Electron Transport Electrophoresis Glucose/analysis,metabolism Lipopolysaccharides/analysis,biosynthesis Lysogeny Mutation Nucleosides/pharmacology Paper Phosphorus Isotopes Rhamnose/analysis Salmonella/analysis,immunology,metabolism Salmonella Phages Spectrum Analysis Tritium
Chemicals
Antigens Carbon Isotopes Lipopolysaccharides Nucleosides Phosphorus Isotopes Tritium Glucose Rhamnose
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Wright A
References (21)
21 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.105.3.927-936.1971
Published
1971-03-00
Pages
927-36
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC248520
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.105.3.927-936.1971 PMC Full Text View journal details
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