Preparation of highly labeled (32P)nucleic acids from yeasts; isolation of "denaturable" leucine acceptor transfer RNA.

Science (New York, N.Y.) ·Vol. 172 ·No. 3981 ·1971-04-23 ·Pages 384-5

Kowalski S, Fresco JR

Abstract

Culture conditions were developed that permit efficient incorporation of [(32)P]phosphate into nucleic acids of baker's yeast. RNA sufficiently labeled for sequence determination was obtained in this way. Pure "denaturable" leucine acceptor transfer RNA (tRNA(Leu)(3)) was isolated for this purpose.

MeSH Terms

Chromatography, DEAE-Cellulose Electrophoresis, Disc Leucine/metabolism Methods Nucleic Acid Denaturation Nucleic Acids/biosynthesis Phosphates/metabolism Phosphorus Isotopes RNA, Transfer/analysis,isolation & purification Saccharomyces/metabolism

Chemicals

Nucleic Acids Phosphates Phosphorus Isotopes RNA, Transfer Leucine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Kowalski S

Fresco J R

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.172.3981.384

Published

1971-04-23

Pages

384-5

Language

English

Region

United States

NLM ID

0404511

Subset

External Links

PubMed source DOI: 10.1126/science.172.3981.384 View journal details

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Preparation of highly labeled (32P)nucleic acids from yeasts; isolation of "denaturable" leucine acceptor transfer RNA.

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