Detection of concanavalin A receptors by affinity to peroxidase and iron dextran by scanning and transmission electron microscopy and x-ray microanalysis.

Journal of microscopy ·Vol. 109 ·No. 2 ·1977-03-00 ·Pages 203-9

Baccetti B, Burrini AG

Abstract

A method of cell surface mapping has been developed on spermatozoa. Concanavalin A binding sites have been simultaneously revelaed both by peroxidase DAB reaction and by iron dextran coupling. The areas are examined in scanning and transmission electron microscopes and submitted to electron probe X-ray microanalysis.

MeSH Terms

Affinity Labels/metabolism Animals Binding Sites Cell Membrane/ultrastructure Electron Probe Microanalysis Iron-Dextran Complex/metabolism Male Microscopy, Electron Microscopy, Electron, Scanning Peroxidases/metabolism Protein Binding Rats Receptors, Concanavalin A/analysis,metabolism Receptors, Drug/analysis Spermatozoa/ultrastructure X-Rays

Chemicals

Affinity Labels Receptors, Concanavalin A Receptors, Drug Iron-Dextran Complex Peroxidases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Baccetti B

Burrini A G

Article Info

Journal

Journal of microscopy

Abbr.

J Microsc

ISSN

0022-2720

DOI

10.1111/j.1365-2818.1977.tb01130.x

Published

1977-03-00

Pages

203-9

Language

English

Region

England

NLM ID

0204522

Subset

External Links

PubMed source DOI: 10.1111/j.1365-2818.1977.tb01130.x View journal details

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Detection of concanavalin A receptors by affinity to peroxidase and iron dextran by scanning and transmission electron microscopy and x-ray microanalysis.

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