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PMID: 559780 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Virion RNA polymerases of two salmonid rhabdoviruses.

Journal of virology ·Vol. 22 ·No. 3 ·1977-06-00 ·Pages 839-43

McAllister PE, Wagner RR

Abstract

RNA-dependent RNA polymerases were found to be associated with two salmonid rhabdoviruses: infectious hematopoietic necrosis (IHN) virus and the virus of hemorrhagic septicemia (VHS). The protein composition of these rhabdoviruses closely resembles that of rabies virus rather than that of vesicular stomatitis virus (McAllister and Wagner, 1975). The optimal temperature for in vitro transcription was found to be approximately 18 degrees C for IHN virus and approximately 15 degrees for VHS,, closely approximating optimal temperatures for growth of these viruses in salmonid cells. Unlike vesicular stomatitis virus, manganese ion (1 mM) could be used as a divalent cation substitute for magnesium ion (5 mM). The in vitro transcription products of IHN and VHS viruses hybridized completely to the homologous genome but not at all to the heterologous genome.

MeSH Terms
Animals Cell Line Cell-Free System DNA-Directed RNA Polymerases/metabolism Fish Diseases/microbiology RNA Viruses/enzymology,growth & development,metabolism RNA, Viral/biosynthesis Salmon Temperature Transcription, Genetic Trout Virus Replication
Chemicals
RNA, Viral DNA-Directed RNA Polymerases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
McAllister P E
Wagner R R
References (19)
19 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.22.3.839-843.1977
Published
1977-06-00
Pages
839-43
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC515786
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.22.3.839-843.1977 PMC Full Text View journal details
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