Isolation and properties of the replicase of encephalomyocarditis virus.
The RNA-dependent RNA polymerase (replicase) of encephalomyocarditis (EMC) virus was found to be closely associated with the smooth membranes of infected BHK-21 cells. An RNA-dependent EMC replicase was extracted from the membranes with 0.15% sodium dodecyl sulfate (SDS) and 1,1,2-trichlorotri-fluoroethane (Genetron 113) and further purified by high-salt dextran-polyethylene glycol phase separation, sievorptive chromatography, and glycerol gradient sedimentation. The enzyme does not manifest strict specificity toward EMC RNA template. It can use also Qbeta RNA, rRNA of BHK cells, or poly(C). SDS-polyacrylamide gel electrophoresis of purified EMC replicase labeled with radioactive methionine revealed that, of all the stable EMC proteins, the enzyme contains predominantly the 56,000-dalton (E) polypeptide.
26 references, click to expand
-
Patterns of macromolecular synthesis in normal and virus-infected mammalian cells.Cold Spring Harb Symp Quant Biol. 1962;27:175-98 PMID: 13959272
-
Preliminary data on a virus-specific enzyme system responsible for the synthesis of viral RNA.Biochem Biophys Res Commun. 1962 Nov 27;9:388-92 PMID: 13966258
-
Induction by an RNA phage of an enzyme catalyzing incorporation of ribonucleotides into ribonucleic acid.Proc Natl Acad Sci U S A. 1963 Mar 15;49:407-14 PMID: 13999778
-
The biosynthesis of ribonucleic acid following infection with a ribonucleic acid virus.J Biol Chem. 1963 May;238:1788-92 PMID: 14021767
-
AN RNA "REPLICASE" INDUCED BY AND SELECTIVE FOR A VIRAL RNA: ISOLATION AND PROPERTIES.Proc Natl Acad Sci U S A. 1963 Nov;50:905-11 PMID: 14082356
-
STUDIES ON EMC VIRAL RNA SYNTHESIS AND ITS LOCALIZATION IN INFECTED KREBS ASCITES CELLS.Virology. 1965 Jul;26:450-65 PMID: 14319717
-
Protein measurement with the Folin phenol reagent.J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
-
Stable polypeptides associated with the 250S mengovirus-induced RNA polymerase structure.Arch Virol. 1975;47(3):201-15 PMID: 164160
-
Symposium on replication of viral nucleic acids. 3. Replication of mengovirus ribonucleic acid.Bacteriol Rev. 1966 Jun;30(2):288-308 PMID: 4287031
-
Evidence for large precursor proteins in poliovirus synthesis.Proc Natl Acad Sci U S A. 1968 Mar;59(3):966-71 PMID: 4296044
-
Polypeptide cleavages in the formation of poliovirus proteins.Proc Natl Acad Sci U S A. 1968 Sep;61(1):77-84 PMID: 4301595
-
Soluble RNA polymerase complex from poliovirus-infected HeLa cells.Virology. 1970 Apr;40(4):840-6 PMID: 4317360
-
The role of cytoplasmic membranes in poliovirus biosynthesis.Virology. 1970 Sep;42(1):100-11 PMID: 4318974
-
Virus-specific proteins synthesized in encephalomyocarditis virus-infected HeLa cells.Proc Natl Acad Sci U S A. 1971 Dec;68(12):3083-7 PMID: 4332006
-
Simple method for the isolation of encephalomyocarditis virus ribonucleic acid.J Virol. 1972 Mar;9(3):559-61 PMID: 4335665
-
Isolation and subunit structure of polycytidylate-dependent RNA polymerase of encephalomyocarditis virus.Proc Natl Acad Sci U S A. 1972 Dec;69(12):3815-9 PMID: 4345509
-
Studies on the replicase of encephalomyocarditis virus.Adv Exp Med Biol. 1974 Jun;44(1):295-308 PMID: 4366015
-
Isolation of a viral polypeptide associated with poliovirus RNA polymerase.Proc Natl Acad Sci U S A. 1974 Dec;71(12):4773-7 PMID: 4373729
-
A new method for the purification of Q RNA-dependent RNA polymerase.Biochim Biophys Acta. 1972 Feb 23;262(1):88-100 PMID: 4552904
-
Gradient sievorptive chromatography. A focusing system for the separation of cellular components.Biochemistry. 1973 Sep 11;12(19):3627-32 PMID: 4596145
-
Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels.Biochem Biophys Res Commun. 1967 Sep 7;28(5):815-20 PMID: 4861258
-
The dissociation of Q-beta-replicase and the relation of one of the components to a poly-C-dependent poly-G-polymerase.Proc Natl Acad Sci U S A. 1967 Jun;57(6):1833-40 PMID: 5231415
-
Structure of the poly(G) polymerase component of the bacteriophage f2 replicase.Proc Natl Acad Sci U S A. 1971 Aug;68(8):1838-43 PMID: 5288771
-
Subunit structure of Q-beta replicase.Nature. 1970 Nov 7;228(5271):525-7 PMID: 5472467
-
Characterization of the subunits of Q-beta replicase.Nature. 1970 Nov 7;228(5271):527-33 PMID: 5472468
-
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.J Biol Chem. 1969 Aug 25;244(16):4406-12 PMID: 5806584
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: [email protected]
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
