Antigen binding to cells: determination by enzymic fluorogenic group hydrolysis.

Science (New York, N.Y.) ·Vol. 159 ·No. 3817 ·1968-02-23 ·Pages 884-5

Sercarz EE, Modabber F

Abstract

A sensitive method for detecting cells containing antibody to beta-galactosidase has been devised. The enzyme attached to the cells containing antibody can hydrolyze a fluorogenic substrate and yield fluorescent products which are measured microphotofluorometrically. This method of detecting a few molecules of antibody is applicable to other enzyme antigen systems.

MeSH Terms

Animals Antigen-Antibody Reactions Fluoresceins Fluorometry Galactosidases Glycosides Immunoassay Mice Photometry Spleen/cytology,enzymology,immunology

Chemicals

Fluoresceins Glycosides Galactosidases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Sercarz E E

Modabber F

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.159.3817.884

Published

1968-02-23

Pages

884-5

Language

English

Region

United States

NLM ID

0404511

Subset

External Links

PubMed source DOI: 10.1126/science.159.3817.884 View journal details

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Antigen binding to cells: determination by enzymic fluorogenic group hydrolysis.

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