Electrical potential and short circuit current of an in vitro preparation of rat colon mucosa.

1. Using a preparation of rat colon mucosa mounted in vitro in small chambers, some factors which influence the electrical properties of the mucosa have been investigated.2. The mucosa behaved mainly as an ohmic resistance although a very brief transient occurred on first passing current. At 32 degrees C, the fresh preparation had a mean resistance of 108Omega/cm(2) and a mean short circuit current (s.c.c.) of 143 muA/cm(2). Tissues taken from Na-depleted and adrenalectomized rats differed little from normal tissues in electrical resistance but those from Na-depleted rats had higher potential difference (p.d.) and s.c.c.3. Increase of temperature led to a rise of conductance of similar order to that found for ions in aqueous solution. S.c.c. also rose with increase of temperature but the effect was relatively greater consistent with its being dependent on metabolic processes.4. Anoxia or the addition of cyanide, iodoacetate or 2,4-dinitrophenol to the bath fluid caused considerable fall in the p.d. and s.c.c.5. Ouabain decreased the p.d. and s.c.c. when added to the serosal side but had no effect when on the luminal side.6. Aldosterone and acetazolamide had no effect.7. Varying serosal side [K] produced only minor changes in p.d.8. Reducing [Na] of the luminal solution caused a considerable fall of p.d. but similar reduction of [Na] on the serosal side had little effect.9. The frequently employed model which represents the transepithelial p.d. as the sum of diffusion potentials originating at the luminal and serosal sides of the cell layer is not consistent with the present results. The colonic transmucosal p.d. probably originates in the electrogenic transport of Na by a mechanism located on the serosal side of the epithelium.