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PMID: 5658585 Published · ppublish English Journal Article

Effect of dietary fat on the fecal excretion of cholesterol and its degradation products in man.

The Journal of clinical investigation ·Vol. 47 ·No. 7 ·1968-07-00 ·Pages 1517-34

Moore RB, Anderson JT, Taylor HL, Keys A, Frantz ID

Abstract

Fecal bile acid and neutral sterol excretion rates were determined in five healthy young men when serum cholesterol changes were induced by isocaloric substitution of an unsaturated (safflower oil) for a saturated fat (butter). The isotope balance method was used after the intravenous injection of cholesterol-4-(14)C. A feces extraction method is presented which permits essentially complete separation of fecal neutral sterols and bile acids. There was a significant increase in the total excretion of the fecal end products of cholesterol metabolism from 966 +/- 42 mg/day on saturated fat to 1147 +/- 45 mg/day on unsaturated fat, and the increase was equally distributed between the neutral sterol and bile acid fractions. With the substitution of dietary fats, regardless of the sequence of their feeding, there was a 28% reduction in serum cholesterol concentration during ingestion of the unsaturated fat. There were reciprocal changes in serum cholesterol levels and fecal steroid excretion with the substitution of one type of fat for the other. The changes in plasma cholesterol content were more than adequately balanced by the reciprocal changes in fecal cholesterol end product excretion. The findings in this study agree with several previous reports in supporting the hypothesis that the hypocholesteremic action of dietary unsaturated fatty acids is associated with an increase in the fecal loss of bile acids and neutral sterols.

MeSH Terms
Adult Anticholesteremic Agents/pharmacology Bile Acids and Salts/analysis Butter/metabolism Carbon Isotopes Cholesterol/blood,metabolism Dietary Fats Fatty Acids, Essential/metabolism Fatty Acids, Nonesterified/metabolism Feces/analysis Humans Male Metabolic Clearance Rate/drug effects Sterols/analysis
Chemicals
Anticholesteremic Agents Bile Acids and Salts Carbon Isotopes Dietary Fats Fatty Acids, Essential Fatty Acids, Nonesterified Sterols Butter Cholesterol
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Moore R B
Anderson J T
Taylor H L
Keys A
Frantz I D
References (73)
73 references, click to expand
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI105845
Published
1968-07-00
Pages
1517-34
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC297311
Subset
IM
External Links
PubMed source DOI: 10.1172/JCI105845 PMC Full Text View journal details
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