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PMID: 5660631 Published · ppublish English Journal Article

Determination of bilirubin glucuronide and assay of glucuronyltransferase with bilirubin as acceptor.

The Biochemical journal ·Vol. 107 ·No. 4 ·1968-04-00 ·Pages 507-18

Van Roy FP, Heirwegh KP

Abstract

1. Conjugated bilirubin is conveniently determined by coupling with the diazonium salt of ethyl anthranilate. 2. This method has been used in the development of assays for UDP-glucuronyltransferase (EC 2.4.1.17), with bilirubin as substrate, in rat liver homogenates, microsomal preparations and partly purified fractions. 3. Chromatographic analysis suggests that bilirubin monoglucuronide is the product of the enzyme systems studied.

MeSH Terms
Animals Azo Compounds Bilirubin/analysis Cattle Diazonium Compounds Glucosyltransferases/analysis Glucuronates/analysis Glycols Humans Hydrogen-Ion Concentration Liver Magnesium Mitochondria, Liver Rats Serum Albumin Spectrophotometry Time Factors ortho-Aminobenzoates
Chemicals
Azo Compounds Diazonium Compounds Glucuronates Glycols Serum Albumin ortho-Aminobenzoates Glucosyltransferases Magnesium Bilirubin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Van Roy F P
Heirwegh K P
References (23)
23 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1070507
Published
1968-04-00
Pages
507-18
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1198694
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1070507 PMC Full Text View journal details
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