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PMID: 5662017 Published · ppublish English Journal Article

Abnormalities of in vitro lymphocyte responses during rubella virus infections.

The Journal of experimental medicine ·Vol. 128 ·No. 1 ·1968-07-01 ·Pages 47-68

Olson GB, Dent PB, Rawls WE, South MA, Montgomery JR, Melnick JL, Good RA

Abstract

In vitro rubella virus infections of lymphocytes from normal adult humans impaired their responsiveness to phytohemagglutinin (PHA) stimulations; a situation which seemed analogous to the PHA unresponsiveness of peripheral lymphocytes from babies with the congenital rubella syndrome. Such in vitro viral infection of normal cells also decreased the synthesis of normal nucleic acids and structural proteins, and abrogated the enhanced DNA synthesis induced by pokeweed and specific antigen stimulations. Furthermore, it was shown that live rubella virus, but not ultraviolet-irradiated virus, was necessary for the impaired mitogenic responses of normal leukocytes. These observations are interpreted to favor the view that the virus achieves its inhibitory effect on the action of mitogens by interference either directly or indirectly at an intracellular site. Such an action could reduce the functional potential of lymphocytes and impair their effectiveness as immunologically competent cells or as effectors in immunologic reactions.

MeSH Terms
Antibody Formation Binding Sites DNA Replication/drug effects Diphtheria Toxoid Hemagglutination Inhibition Tests Humans Infant Infant, Newborn Lectins Lymphocytes/immunology,metabolism Neutralization Tests RNA/biosynthesis Rubella/congenital,immunology Rubella virus/immunology Tetanus Toxoid
Chemicals
Diphtheria Toxoid Lectins Tetanus Toxoid RNA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Olson G B
Dent P B
Rawls W E
South M A
Montgomery J R
Melnick J L
Good R A
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47 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.128.1.47
Published
1968-07-01
Pages
47-68
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2138505
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.128.1.47 PMC Full Text View journal details
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