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PMID: 5673936 Published · ppublish English Journal Article

The effects of catabolic and anabolic steroids on amino acid incorporation by skeletal-muscle ribosomes.

The Biochemical journal ·Vol. 108 ·No. 3 ·1968-07-00 ·Pages 417-25

Bullock G, White AM, Worthington J

Abstract

1. A method is described for the routine isolation of ribosomes from small quantities of skeletal muscle that have been homogenized with the Ultra-Turrax tissue disintegrator. 2. Ribosomes prepared by this method from rats receiving triamcinolone acetonide or rabbits receiving cortisone acetate show a marked fall in their ability to incorporate amino acids when compared with ribosomes from control animals. 3. This fall in activity can be partially prevented in rats by pretreating the animals with an anabolic steroid, steroid 36644-Ba. 4. Testosterone (5mg./kg.) administered to rabbits in conjunction with cortisone acetate is not effective in maintaining ribosomal activity. However, steroid 36644-Ba at one-tenth of an equiandrogenic dose (0.05mg./kg.) is extremely effective. 5. The results with ribosomes isolated from rabbits support the concept that steroid 36644-Ba and possibly all anabolic steroids have an ability to counteract the catabolic action of corticosteroids that is greater than their androgenic activity would suggest.

MeSH Terms
Amino Acids/metabolism Anabolic Agents/pharmacology Animals Carbon Isotopes Centrifugation, Density Gradient Female Leucine/metabolism Male Methods Muscles/metabolism RNA/analysis Rabbits Rats Ribosomes/metabolism Steroids/pharmacology Time Factors
Chemicals
Amino Acids Anabolic Agents Carbon Isotopes Steroids RNA Leucine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bullock G
White A M
Worthington J
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19 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1080417
Published
1968-07-00
Pages
417-25
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1198827
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1080417 PMC Full Text View journal details
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