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PMID: 5688926 Published · ppublish English Journal Article

The distribution of glutamate decarboxylase and aspartate transaminase in subcellular fractions of rat and guinea-pig brain.

The Biochemical journal ·Vol. 106 ·No. 2 ·1968-01-00 ·Pages 401-12

Fonnum F

Abstract

1. The subcellular distributions of glutamate decarboxylase and aspartate transaminase were studied in rat and guinea-pig brain. 2. Glutamate decarboxylase is localized in the synaptosome fraction. The mean density of the particles containing the enzyme is slightly greater than those derived from cholinergic neurones, though overlap is substantial. 3. The enzyme is readily released from synaptosomes by hypo-osmotic treatment, but in the presence of Ca(2+), Na(+) and K(+) it sediments with particulate material. 4. The release and binding of the enzyme to membrane fractions by Ca(2+) were investigated. 5. Aspartate transaminase is present in brain as two isoenzymes with different kinetic properties. One isoenzyme is associated with the cytoplasm and the other with mitochondria.

MeSH Terms
Acetylcholine/analysis Acyltransferases/analysis Animals Aspartate Aminotransferases/analysis Brain/cytology,enzymology Carboxy-Lyases/analysis Centrifugation, Density Gradient Cytoplasm/enzymology Guinea Pigs Hydro-Lyases/analysis Isoenzymes/analysis Kinetics Mitochondria/enzymology Rats
Chemicals
Isoenzymes Acyltransferases Aspartate Aminotransferases Carboxy-Lyases Hydro-Lyases Acetylcholine
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Fonnum F
References (29)
29 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1060401
Published
1968-01-00
Pages
401-12
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1198516
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1060401 PMC Full Text View journal details
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