Home LiteratureArticle Details
PMID: 569290 Published · ppublish English Journal Article

Localisation of foldback DNA sequences in nuclei chromosomes of Scilla, Secale, and of mouse.

Nucleic acids research ·Vol. 5 ·No. 10 ·1978-10-00 ·Pages 3589-602

Deumling B

Abstract

Foldback DNA, prepared from mouse and Scilla sibirica main band DNA, and from rye (Secale cereale) total DNA, was characterised by denaturation, renaturation, and electron microscopy. 3H-cRNA of this DNA was hybridised in situ to nuclei and chromosomes of the respective species. There is no universal labelling pattern among the three species. In mouse, highly repetitive foldback DNA is present in the whole chromatin including the satellite DNA-containing regions. In Scilla sibirica, on the contrary, the highly repetitive foldback sequences are excluded form the satellite DNA loci and are arranged in clusters in the remaining chromatin. In rye, there is a clear preferential labelling of the chromocenters in the interphase nuclei as well as metaphase chromosomes, indicating that highly repetitive foldback DNA is preferentially located among other highly repetitive sequences.

MeSH Terms
Animals Base Sequence Chromosomes/ultrastructure DNA/genetics Kinetics Mice/genetics Nucleic Acid Renaturation Plants/genetics Plants, Medicinal/genetics
Chemicals
DNA
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Deumling B
References (21)
21 references, click to expand
  1. Initiation of adenovirus DNA replication does not occur via a hairpin mechanism.
    Nucleic Acids Res. 1978 Apr;5(4):1289-95 PMID: 148639
  2. Giemsa C-banding and the evolution of wheat.
    Proc Natl Acad Sci U S A. 1974 Oct;71(10):4086-90 PMID: 16592188
  3. Characterization of foldback sequences in Physarum polycephalum nuclear DNA using the electron microscope.
    Eur J Biochem. 1977 Apr 1;74(2):275-83 PMID: 558095
  4. Characterization of inverted repeated sequences in wheat nuclear DNA.
    Nucleic Acids Res. 1978 Mar;5(3):751-69 PMID: 643618
  5. Inverted repeat sequences in the Drosophila genome.
    Cell. 1975 Jun;5(2):159-72 PMID: 806348
  6. Effect of different denaturing agents on the detectability of specific DNA sequences of various base compositions by in situ hybridisation.
    Chromosoma. 1977 Apr 20;60(4):377-89 PMID: 858264
  7. Characterization of foldback sequences in hamster DNA using electron microsocpy.
    Nucleic Acids Res. 1977 Jan;4(1):247-68 PMID: 866177
  8. The organization of DNA sequences in the mouse genome.
    Chromosoma. 1977 May 23;61(3):215-26 PMID: 872701
  9. Inverted repetitive sequences in the human genome.
    Biochemistry. 1976 Sep 7;15(18):4120-5 PMID: 963027
  10. An electron microscope study of the DNA sequence organization of the human genome.
    J Mol Biol. 1976 Sep 25;106(3):773-90 PMID: 988187
  11. An electron microscopic study of mouse foldback DNA.
    Cell. 1975 Aug;5(4):429-46 PMID: 1157097
  12. Localisation of satellite DNA sequences in nuclei and chromosomes of two plants.
    Nature. 1975 Sep 11;257(5522):152-5 PMID: 1161019
  13. On inverted repeat sequences in chromosomal DNA.
    Genetics. 1976 Jan;82(1):139-40 PMID: 1248734
  14. Granulated hydroxyapatite: preparation and chromatographic properties.
    Anal Biochem. 1974 Sep;61(1):62-71 PMID: 4370057
  15. Palindromic base sequences and replication of eukaryote chromosome ends.
    Nature. 1974 Aug 9;250(5466):467-70 PMID: 4469597
  16. Double-stranded regions in heterogeneous nuclear RNA from Hela cells.
    Proc Natl Acad Sci U S A. 1972 Sep;69(9):2537-41 PMID: 4506771
  17. Improved conditions for in situ RNA-DNA hybridization.
    FEBS Lett. 1973 Apr 1;31(1):85-8 PMID: 4710003
  18. Double-helical regions in nuclear precursor of mRNA (pre-mRNA).
    Biochim Biophys Acta. 1973 Jun 8;312(1):152-64 PMID: 4737856
  19. A low-viscosity epoxy resin embedding medium for electron microscopy.
    J Ultrastruct Res. 1969 Jan;26(1):31-43 PMID: 4887011
  20. A study in evolution: the DNA base sequence homology between coliphages T7 and T3.
    J Mol Biol. 1971 Dec 14;62(2):287-301 PMID: 4944929
  21. Inverted sequences in rat DNA.
    FEBS Lett. 1977 May 1;77(1):94-8 PMID: 404193
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/5.10.3589
Published
1978-10-00
Pages
3589-602
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342697
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/5.10.3589 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2