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PMID: 5693925 Published · ppublish English Journal Article

Cytotoxicity mediated by soluble antigen and lymphocytes in delayed hypersensitivity. 3. Analysis of mechanism.

The Journal of experimental medicine ·Vol. 128 ·No. 6 ·1968-12-01 ·Pages 1267-79

Ruddle NH, Waksman BH

Abstract

The cytopathic effect of lymph node cells from tuberculin-sensitized rats on rat embryo fibroblasts in the presence of PPD was not enhanced by admixture of normal (nonsensitized) lymph node cells. Preincubation studies showed that this in vitro response is initiated by the reaction of lymphocytes with specific antigen, beginning within 30 min, rather than uptake of antigen by the fibroblasts. The supernatant fluids from suspensions of sensitized cells incubated with PPD for 17 hr or more possessed cytotoxic activity. The target fibroblasts showed a marked increase in acid phosphatase content within 48 hr after the addition of sensitized lymph node cells and antigen.

MeSH Terms
Acid Phosphatase/analysis Animals Bacterial Proteins Culture Techniques Fibroblasts/analysis,immunology Histocytochemistry Hypersensitivity, Delayed/immunology Lymphocytes/immunology Rats Time Factors Tuberculin
Chemicals
Bacterial Proteins Tuberculin Acid Phosphatase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ruddle N H
Waksman B H
References (31)
31 references, click to expand
  1. Cytotoxicity mediated by soluble antigen and lymphocytes in delayed hypersensitivity. I. Characterization of the phenomenon.
    J Exp Med. 1968 Dec 1;128(6):1237-54 PMID: 4176937
  2. STUDIES ON RABBIT LYMPHOCYTES IN VITRO. I. STIMULATION OF BLAST TRANSFORMATION WITH AN ANTIALLOTYPE SERUM.
    J Exp Med. 1965 Aug 1;122:423-40 PMID: 14316952
  3. Lymphocyte RNA metabolism. Comparison between antigen and phytohaemagglutinin stimulation.
    Lancet. 1965 Oct 9;2(7415):723-4 PMID: 4157780
  4. TISSUE CULTURE STUDIES OF THE HUMAN LYMPHOCYTE.
    Science. 1964 Dec 25;146(3652):1648-54 PMID: 14224511
  5. IN VITRO DEMONSTRATION OF ANTI-OVALBUMIN SPECIFICITY OF LYMPH NODE CELLS IN DELAYED TYPE HYPERSENSITIVITY.
    J Immunol. 1963 Mar;90:337-46 PMID: 14051867
  6. Studies on the mechanism of delayed type hypersensitivity in tissue cultures. IX. The effect of glucose concentration on the reaction of delayed type hypersensitivity in tissue cultures.
    Z Immunitatsforsch Allerg Klin Immunol. 1967 Apr;132(4):378-94 PMID: 4232422
  7. THE DEMONSTRATION OF A PHENOMENON IN VITRO APPLICABLE TO THE STUDY OF DELAYED HYPERSENSITIVITY.
    J Allergy. 1963 Jul-Aug;34:337-47 PMID: 14046405
  8. Mechanism of a reaction in vitro associated with delayed-type hypersensitivity.
    Science. 1966 Jul 1;153(3731):80-2 PMID: 5938421
  9. Suppression of delayed hypersensitivity in vitro by inhibition of protein synthesis.
    J Exp Med. 1965 Dec 1;122(6):1125-34 PMID: 5853077
  10. GAMMA-GLOBULIN PRODUCTION BY HUMAN LYMPHOCYTES IN VITRO.
    Exp Cell Res. 1963 Dec;32:592-5 PMID: 14099828
  11. Cell-bound antibodies in transplantation immunity.
    Ann N Y Acad Sci. 1958 Oct 7;73(3):654-62 PMID: 13617873
  12. IMMUNE RESPONSE AND MITOSIS OF HUMAN PERIPHERAL BLOOD LYMPHOCYTES IN VITRO.
    Science. 1963 Nov 29;142(3596):1185-7 PMID: 14069243
  13. Some quantitative aspects of the uptake of antigens in vitro by the lymphocytes of hypersensitive guinea pigs.
    Int Arch Allergy Appl Immunol. 1960;17:338-51 PMID: 13778697
  14. The effect of glucose on the tuberculin reaction in tissue culture.
    Am Rev Tuberc. 1958 Nov;78(5):712-24 PMID: 13583435
  15. Macrophage migration.
    Fed Proc. 1968 Jan-Feb;27(1):6-12 PMID: 4952935
  16. Cellular immunity.
    Prog Allergy. 1967;11:89-140 PMID: 4860381
  17. Cytotoxic potential of stimulated human lymphocytes.
    J Exp Med. 1967 Apr 1;125(4):721-36 PMID: 6020009
  18. RNA METABOLISM IN LYMPHOCYTES STIMULATED BY PHYTOHEMAGGLUTININ: INITIAL RESPONSES TO PHYTOHEMAGGLUTININ.
    Blood. 1965 Jun;25:1014-27 PMID: 14294763
  19. Tuberculin hypersensitivity: studies with radioactive antigen and mononuclear cells.
    Science. 1963 Feb 8;139(3554):487-90 PMID: 14031568
  20. Delayed hypersensitivity in man: a correlate in vitro and transfer by an RNA extract.
    Science. 1967 Sep 29;157(3796):1567-9 PMID: 6038169
  21. THE IN VITRO DESENSITIZATION OF SENSITIVE CELLS BY TRYPSIN.
    J Exp Med. 1964 Dec 1;120:1189-200 PMID: 14238934
  22. Studies on the migration inhibitory factor associated with delayed-type hypersensitivity: cytodynamics and specificity.
    Transplantation. 1967 Jul;5(4):Suppl:996-1000 PMID: 6049785
  23. Leukocytes and interferon in the host response to viral infections. II. Enhanced interferon response of leukocytes from immune animals.
    J Bacteriol. 1966 Jun;91(6):2185-91 PMID: 5943935
  24. Release of an endogenous pyrogen in vitro from rabbit mononuclear cells.
    J Exp Med. 1967 Aug 1;126(2):357-84 PMID: 6028491
  25. Reactions in vivo and in vitro produced by a soluble substance associated with delayed-type hypersensitivity.
    Proc Natl Acad Sci U S A. 1968 Mar;59(3):756-62 PMID: 5238658
  26. Quantitative studies on the behavior of sensitized lymphoid cells in vitro. 3. Conversion of "normal" lymphoid cells to an immunologically active status with RNA derived from isologous lymphoid tissues of specifically immunized rats.
    J Immunol. 1966 Oct;97(4):512-6 PMID: 5957257
  27. Evolving patterns of RNA metabolism during transition from resting state to active growth in lymphocytes stimulated by phytohemagglutinin.
    Proc Natl Acad Sci U S A. 1965 Aug;54(2):469-76 PMID: 5217435
  28. ACID PHOSPHATASE-RICH GRANULES IN HUMAN LYMPHOCYTES INDUCED BY PHYTOHEMAGGLUTININ.
    Science. 1965 Jan 1;147(3653):55-7 PMID: 14224525
  29. The effect of tuberculin on peritoneal exudate cells of sensitized guinea pigs in surviving cell culture.
    J Immunol. 1958 Sep;81(3):220-34 PMID: 13575818
  30. DELAYED HYPERSENSITIVITY IN VITRO. II. EFFECT OF SENSITIVE CELLS ON NORMAL CELLS IN THE PRESENCE OF ANTIGEN.
    J Immunol. 1964 Aug;93:274-8 PMID: 14202256
  31. Cytotoxicity mediated by soluble antigen and lymphocytes in delayed hypersensitivity. II. Correlation of the in vitro response with skin reactivity.
    J Exp Med. 1968 Dec 1;128(6):1255-65 PMID: 4235040
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.128.6.1267
Published
1968-12-01
Pages
1267-79
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2138574
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.128.6.1267 PMC Full Text View journal details
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