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PMID: 574514 Published · ppublish English Journal Article

High resolution scanning electron microscopy of isolated and in situ cytoskeletal elements.

The Journal of cell biology ·Vol. 83 ·No. 1 ·1979-10-00 ·Pages 249-54

Ip W, Fischman DA

Abstract

Evidence is presented that cytoskeletal structures (actin filaments, intermediate filaments, and microtubules) can be resolved by scanning electron microscopy after osmium impregnation of biological material, using thiocarbohydrazide as a ligand, followed by critical-point drying. These different classes of filaments or tubules can be identified both as purified protein polymers and as structured organelles within cryofractured or detergent-extracted cells.

MeSH Terms
Actins Animals Brain/ultrastructure Chickens/anatomy & histology Cytoskeleton/ultrastructure Microscopy, Electron Microscopy, Electron, Scanning Microtubules/ultrastructure Muscle, Smooth/ultrastructure Muscles/ultrastructure Rabbits Swine/anatomy & histology
Chemicals
Actins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ip W
Fischman D A
References (19)
19 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.83.1.249
Published
1979-10-00
Pages
249-54
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2110435
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.83.1.249 PMC Full Text View journal details
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