Replicative intermediate of an arbovirus.

Journal of virology ·Vol. 2 ·No. 6 ·1968-06-00 ·Pages 547-52

Friedman RM

Abstract

One hour after infection of chick fibroblasts with Semliki Forest virus (SFV), a viral ribonucleic acid (RNA) structure is present which has many of the properties described for the replicative intermediate of several RNA bacteriophages. These properties include a polydisperse nature on sucrose density gradient analysis, ribonuclease resistance, a variation in sedimentation pattern associated with changes in salt concentration, and recovery of infectious viral RNA upon denaturation. Most of the replicative intermediate present in SFV infection appears to be membrane-associated.

MeSH Terms

Animals Bacteriophages Centrifugation, Density Gradient Chick Embryo Culture Techniques Cytoplasm Fibroblasts RNA, Viral/metabolism Semliki forest virus Tritium Virus Replication

Chemicals

RNA, Viral Tritium

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Friedman R M

References (16)

16 references, click to expand

Protein synthesis directed by an arbovirus.
J Virol. 1968 Jan;2(1):26-32 PMID: 5754569
Replication of viral RNA, 8. Studies on the enzymatic mechanism of replication of MS2 RNA.
Proc Natl Acad Sci U S A. 1965 Sep;54(3):982-7 PMID: 5217476
The reaction of EEE virus preparations with sodium deoxycholate.
Virology. 1963 Jun;20:263-8 PMID: 13982184
The solvent denaturation of double-stranded RNA from poliovirus infected HeLa cells.
Biochem Biophys Res Commun. 1966 May 25;23(4):557-60 PMID: 4289760
The effect of interferon on the synthesis of RNA in chick cells infected with Semliki forest virus.
J Gen Virol. 1967 Jan;1(1):25-40 PMID: 6060240
AN IMPROVED PROCEDURE FOR MEASURING THE DISTRIBUTION OF P32O4--AMONG THE NUCLEOTIDES OF RIBONUCLEIC ACID.
Anal Biochem. 1964 May;8:126-9 PMID: 14167265
Cytoplasmic fractions associated with Semliki Forest virus ribonucleic acid replication.
J Virol. 1967 Apr;1(2):374-83 PMID: 5630381
Ribonucleic acid polymerase catalyzing synthesis of double-stranded arbovirus ribonucleic acid.
J Virol. 1967 Feb;1(1):97-109 PMID: 5623960
A NEW RNA COMPONENT IN MS2-INFECTED CELLS.
J Mol Biol. 1964 Apr;8:602-5 PMID: 14153530
Infectious nucleic acids of e. Coli bacteriophages, ix. Sedimentation constants and strand integrity of infectious m12 phage replicative-form RNA.
Proc Natl Acad Sci U S A. 1966 Dec;56(6):1883-90 PMID: 16591434
An intermediate in the synthesis of poliovirus RNA.
Proc Natl Acad Sci U S A. 1966 Aug;56(2):741-8 PMID: 4291014
REPLICATION OF THE RNA OF BACTERIOPHAGE R17.
Science. 1964 Oct 23;146(3643):527-30 PMID: 14190242
Replication of semliki forest virus: three forms of viral RNA produced during infection.
Proc Natl Acad Sci U S A. 1966 Aug;56(2):440-6 PMID: 5229967
Sedimentation characteristics of rapidly labelled RNA from HeLa cells.
Biochem Biophys Res Commun. 1962 Jun 4;7:486-90 PMID: 14498283
Viral specific RNAs in infected cells.
Nature. 1967 Jan 28;213(5074):365-7 PMID: 6067598
On the structure and replication of influenza virus.
J Mol Biol. 1967 May 14;25(3):383-405 PMID: 6068145

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.2.6.547-552.1968

Published

1968-06-00

Pages

547-52

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC375647

Subset

External Links

PubMed source DOI: 10.1128/JVI.2.6.547-552.1968 PMC Full Text View journal details

Analysis Services

Replicative intermediate of an arbovirus.

Contact

WeChat Official Account