Home LiteratureArticle Details
PMID: 5764347 Published · ppublish English Journal Article

Genetic relationship between Sarcina ureae and members of the genus Bacillus.

Journal of bacteriology ·Vol. 97 ·No. 1 ·1969-01-00 ·Pages 6-12

Herndon SE, Bott KF

Abstract

Deoxyribonucleic acid-ribonucleic acid (DNA-RNA) and DNA-DNA hybridization studies were performed to determine the degree of genetic relatedness between Sarcina ureae and several members of the genus Bacillus. DNA-RNA hybridization showed a high degree of homology between S. ureae RNA and DNA from Bacillus species having a similar guanine plus cytosine content. The DNA from other genera of the family Micrococcaceae showed less homology with S. ureae RNA than did that of the Bacillus species tested; however, this homology was not found between the DNA of S. ureae and DNA from these Bacillus species or DNA from the other Micrococcaceae tested. Transformation with Bacillus DNA, infection with representatives from several major classes of Bacillus phages, and electrophoretic analysis of proteins in crude extracts of these strains were also attempted as a further test of the genetic relationship between the genera. These experiments did not support the belief that the two groups are closely related genetically.

MeSH Terms
Bacillus/classification Bacterial Proteins/analysis Bacteriophages Cytosine/analysis DNA, Bacterial/analysis Electrophoresis Genetics, Microbial Guanine/analysis Hot Temperature Micrococcus/classification RNA, Bacterial/analysis Sarcina/classification Transformation, Genetic
Chemicals
Bacterial Proteins DNA, Bacterial RNA, Bacterial Guanine Cytosine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Herndon S E
Bott K F
References (20)
20 references, click to expand
  1. Taxonomy of Sarcina on the basis of their DNA base composition.
    Acta Biochim Pol. 1967;14(1):31-40 PMID: 6049226
  2. A general method for the isolation of RNA complementary to DNA.
    Proc Natl Acad Sci U S A. 1962 Aug;48:1390-7 PMID: 13870855
  3. Studies on hybrid molecules of nucleic acids. I. DNA-DNA hybrids on nitrocellulose filters.
    Biochem Biophys Res Commun. 1967 Aug 23;28(4):550-7 PMID: 6052490
  4. Electron Microscopy of Ultrathin Sections of Sporosarcina ureae.
    J Bacteriol. 1965 Sep;90(3):808-16 PMID: 16562085
  5. GERMINATION SYSTEM FOR ENDOSPORES OF SARCINA UREAE.
    J Bacteriol. 1964 Jan;87:235-6 PMID: 14102865
  6. Deoxyribonucleic acid homology and taxonomy of Agrobacterium, Rhizobium, and Chromobacterium.
    J Bacteriol. 1967 Jul;94(1):116-24 PMID: 4961629
  7. An approach to the measurement of genetic relatedness among organisms.
    Proc Natl Acad Sci U S A. 1963 Jul;50:156-64 PMID: 13932048
  8. NEW APPROACHES TO BACTERIAL TAXONOMY.
    Annu Rev Microbiol. 1963;17:329-72 PMID: 14147455
  9. An index to deoxyribonucleic acid base compositions of bacterial species.
    J Gen Microbiol. 1966 Sep;44(3):419-37 PMID: 5339419
  10. Development of competence in the Bacillus subtilis transformation system.
    J Bacteriol. 1967 Sep;94(3):562-70 PMID: 4962301
  11. Deoxyribonucleic acid base composition of some members of the Micrococcaceae.
    J Bacteriol. 1966 Jul;92(1):28-34 PMID: 5941282
  12. On the isolation of dipicolinic acid fromhendospores of Sarcina ureae.
    Arch Mikrobiol. 1963;45:27-32 PMID: 13981202
  13. PREPARATION OF TRANSFORMING DEOXYRIBONUCLEIC ACID BY PHENOL TREATMENT.
    Biochim Biophys Acta. 1963 Aug 20;72:619-29 PMID: 14071565
  14. CONSERVATION OF RIBOSOMAL AND MESSENGER RIBONUCLEIC ACID CISTRONS IN BACILLUS SPECIES.
    J Bacteriol. 1965 Aug;90:384-90 PMID: 14329452
  15. A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane.
    J Mol Biol. 1965 Jul;12(3):829-42 PMID: 4955314
  16. TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE.
    Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072-8 PMID: 16590310
  17. Gene conservation in Bacillus species. I. Conserved genetic and nucleic acid base sequence homologies.
    Proc Natl Acad Sci U S A. 1965 Aug;54(2):491-8 PMID: 4956287
  18. Heterogeneity of the conserved ribosomal ribonucleic acid sequences of Bacillus subtilis.
    J Bacteriol. 1966 Jul;92(1):88-96 PMID: 4957439
  19. POSSIBLE SYNTHESIS OF POLYRIBONUCLEOTIDES OF KNOWN BASE-TRIPLET SEQUENCES.
    Nature. 1965 Apr 3;206:93 PMID: 14334364
  20. Nutritional factors influencing the development of competence in the Bacillus subtilis transformation system.
    J Bacteriol. 1968 Apr;95(4):1439-49 PMID: 4967198
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.97.1.6-12.1969
Published
1969-01-00
Pages
6-12
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC249532
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.97.1.6-12.1969 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2