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PMID: 5770880 Published · ppublish English Journal Article

Osmotic responses demonstrating the extracellular character of the sarcoplasmic reticulum.

The Journal of physiology ·Vol. 202 ·No. 1 ·1969-05-00 ·Pages 171-88

Birks RI, Davey DF

Abstract

1. Changes in the dimensions of the sarcoplasmic reticulum in frog sartorius muscles exposed to hypertonic and hypotonic solutions have been studied with the electron microscope.2. The volume of the sarcoplasmic reticulum has been found to be linearly related with a negative slope to the reciprocal of the osmotic pressure. Over the range 0.75 to 3.5 x normal osmotic pressure the reticulum volume has been calculated to change from 11.5 to 18.5% of normal cell volume.3. These changes in sarcoplasmic reticulum volume correspond to the calculated changes in the volume of the intra-fibre sucrose compartment, postulated by earlier workers on the basis of studies on changes in cell volume with changes in osmotic pressure in living muscles.4. To explain these and other related findings on the distribution of electrolytes in muscle, it is proposed that the sarcoplasmic reticulum of skeletal muscle is an extracellular compartment.5. The significance of this hypothesis for the mechanism of excitation-contraction coupling is discussed.

MeSH Terms
Animals Anura Extracellular Space Hypertonic Solutions Hypotonic Solutions Microscopy, Electron Muscle Contraction Muscles/cytology Myofibrils/physiology Osmosis Osmotic Pressure Sodium Chloride Sucrose/metabolism
Chemicals
Hypertonic Solutions Hypotonic Solutions Sodium Chloride Sucrose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Birks R I
Davey D F
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30 references, click to expand
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Article Info
Journal
The Journal of physiology
Abbr.
J Physiol
ISSN
0022-3751
DOI
10.1113/jphysiol.1969.sp008802
Published
1969-05-00
Pages
171-88
Language
English
Region
England
NLM ID
0266262
PMCID
PMC1351472
Subset
IM
External Links
PubMed source DOI: 10.1113/jphysiol.1969.sp008802 PMC Full Text View journal details
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