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PMID: 5775795 Published · ppublish English Journal Article

The human chromosome. Electron microscopic observations on chromatin fiber organization.

The Journal of cell biology ·Vol. 41 ·No. 1 ·1969-04-00 ·Pages 73-90

Abuelo JG, Moore DE

Abstract

Human lymphocytes were grown in short-term tissue culture and were arrested in metaphase with Colcemid. Their chromosomes were prepared by the Langmuir trough-critical point drying technique and were examined under the electron microscope. In addition, some chromosomes were digested with trypsin, Pronase, or DNase. The chromosomes consist entirely of tightly packed, 240 +/- 50-A chromatin fibers. Trypsin and Pronase treatments induce relaxation of fiber packing and reveal certain underlying fiber arrangements. Furthermore, trypsin treatment demonstrates that the chromatin fiber has a 25-50 A trypsin-resistant core surrounded by a trypsin-sensitive sheath. DNase digestion suggests that this core contains DNA.

MeSH Terms
Adult Chromatids Chromosome Mapping Chromosomes, Human, 6-12 and X Culture Techniques Cytogenetics Female Humans Lymphocytes/cytology Microscopy, Electron
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Abuelo J G
Moore D E
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48 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.41.1.73
Published
1969-04-00
Pages
73-90
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2107724
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.41.1.73 PMC Full Text View journal details
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