Analysis of the diluents used for rubella virus hemagglutination and hemagglutination-inhibition tests.

Applied microbiology ·Vol. 18 ·No. 2 ·1969-08-00 ·Pages 221-7

Dold HJ, Northrop RL

Abstract

A new diluent (ADGP) for the rubella virus hemagglutination (HA) and hemagglutination-inhibition (HI) tests is described. It was found that the HA and HI titers and the erythrocyte agglutination pattern were improved in ADGP compared to previously described diluents. The influence of the components of ADGP and of various test conditions on optimal HA and HI results were examined.

MeSH Terms

Animals Calcium Chloride Chickens Erythrocytes Gelatin Glucose Hemagglutination Inhibition Tests Hemagglutination Tests Magnesium Rubella virus/immunology Serum Albumin, Bovine Sodium Chloride Solutions

Chemicals

Solutions Serum Albumin, Bovine Sodium Chloride Gelatin Magnesium Glucose Calcium Chloride

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Dold H J

Northrop R L

References (9)

9 references, click to expand

Rubella-virus hemagglutination-inhibition test.
N Engl J Med. 1967 Mar 9;276(10):554-7 PMID: 6019765
Rubella hemagglutinin prepared with alkaline extraction of virus grown in suspension culture of BHK-21 cells.
Proc Soc Exp Biol Med. 1967 May;125(1):162-7 PMID: 6027517
[The use of pigeon eruthrocytes in the hemagglutination inhibition test for rubella].
Presse Med. 1967 Oct 21;75(43):2177-8 PMID: 6069552
Is rubella an arbovirus?
Lancet. 1967 Dec 9;2(7528):1233-6 PMID: 4169410
An improved diluent for rubella hemagglutination and hemagglutination-inhibition tests.
Appl Microbiol. 1968 May;16(5):691-4 PMID: 5659363
The nonspecific inhibitors of rubella-virus hemagglutination.
Proc Soc Exp Biol Med. 1968 Jun;128(2):577-81 PMID: 5663277
Heparin-MnCl2 removal of nonspecific serum inhibitor for measles virus hemagglutinin.
J Immunol. 1968 Jun;100(6):1353-4 PMID: 5696290
A simple method for removal of rubella hemagglutination inhibitors from serum adaptable to finger-tip blood.
Am J Epidemiol. 1968 Nov;88(3):301-4 PMID: 4972370
Experience with a modified rubella hemagglutination inhibition antibody test.
JAMA. 1969 Jan 6;207(1):89-93 PMID: 5818144

Article Info

Journal

Applied microbiology

Abbr.

Appl Microbiol

ISSN

0003-6919

DOI

10.1128/am.18.2.221-227.1969

Published

1969-08-00

Pages

221-7

Language

English

Region

United States

NLM ID

7605802

PMCID

PMC377947

Subset

External Links

PubMed source DOI: 10.1128/am.18.2.221-227.1969 PMC Full Text

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