Nitrite oxidase and nitrate reductase in Nitrobacter agilis.

The Biochemical journal ·Vol. 113 ·No. 3 ·1969-07-00 ·Pages 449-55

Faull KF, Wallace W, Nicholas DJ

Abstract

Nitrite oxidase and nitrate reductase in Nitrobacter agilis were shown to be separate enzymes. The best separation of the two systems was achieved by ammonium sulphate fractionation. The effects of various compounds, including antimycin A, 2-n-heptyl-4-hydroxyquinoline N-oxide and chlorate, also clearly distinguish between the two enzyme reactions. The relationship between the two opposing reactions in Nitrobacter is discussed.

MeSH Terms

Antimycin A/pharmacology Centrifugation Chemical Precipitation Nitrates/metabolism Nitrites/metabolism Nitrobacter/enzymology Oxidoreductases/antagonists & inhibitors,isolation & purification Quaternary Ammonium Compounds Quinolines/pharmacology Spectrophotometry

Chemicals

Nitrates Nitrites Quaternary Ammonium Compounds Quinolines Antimycin A Oxidoreductases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Faull K F

Wallace W

Nicholas D J

References (11)

11 references, click to expand

MOLYBDENUM AS A MICRONUTRIENT FOR NITROBACTER.
J Bacteriol. 1965 Jan;89:123-8 PMID: 14255651
BIOLOGICAL OXIDOREDUCTIONS.
Annu Rev Biochem. 1964;33:729-90 PMID: 14268850
The biochemistry of the nitrifying organisms. V. Nitrite oxidation by Nitrobacter.
Biochem J. 1957 Feb;65(2):297-305 PMID: 13403908
Spectrophotometric and kinetic study of nitrite and formate oxidation in Nitrobacter winogradskyi.
J Bacteriol. 1967 Jan;93(1):215-20 PMID: 4289808
PHOSPHORYLATION COUPLED TO NITRITE OXIDATION BY PARTICLES FROM THE CHEMOAUTOTROPH, NITROBACTER AGILIS.
Proc Natl Acad Sci U S A. 1960 Jun;46(6):763-9 PMID: 16590668
Interference by reduced pyridine nucleotides in the diazotization of nitrite.
Biochim Biophys Acta. 1957 Feb;23(2):440-2 PMID: 13412748
CHARACTERIZATION OF A NITRATE REDUCTASE FROM THE CHEMOAUTOTROPH NITROBACTER AGILIS.
J Biol Chem. 1965 Mar;240:1412-26 PMID: 14284757
ON THE ASSIMILATION OF ENERGY FROM INORGANIC SOURCES IN AUTOTROPHIC FORMS OF LIFE.
Proc Natl Acad Sci U S A. 1964 Oct;52:980-8 PMID: 14224403
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Cytochromes of Nitrobacter.
Nature. 1958 Sep 13;182(4637):732-3 PMID: 13590096
The mechanism of nitrite oxidation by Nitrobacter winogradskyi.
Biochim Biophys Acta. 1966 Jan 11;113(1):41-50 PMID: 5940631

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1130449

Published

1969-07-00

Pages

449-55

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1184686

Subset

External Links

PubMed source DOI: 10.1042/bj1130449 PMC Full Text View journal details

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