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PMID: 5874548 Published · ppublish English Journal Article

Role of ribonucleic acid biosynthesis in multiplication of type 5 adenovirus.

Journal of bacteriology ·Vol. 87 ·No. 5 ·1964-05-00 ·Pages 977-87

Flanagan JF, Ginsberg HS

Abstract

Flanagan, John F. (University of Pennsylvania, Philadelphia), and Harold S. Ginsberg. Role of ribonucleic acid biosynthesis in multiplication of type 5 adenovirus. J. Bacteriol. 87:977-987. 1964.-The requirement for ribonucleic acid (RNA) biosynthesis in the multiplication of type 5 adenovirus was investigated by using radioactive phosphorus to label nucleic acids and two pyrimidine analogues, 6-azauridine and 5-fluorouracil or 5-fluorouridine, to inhibit synthesis of functional RNA. The data indicate that biosynthesis of RNA after infection is essential for production of virus-specific deoxyribonucleic acid, virus antigens, and infectious particles. The onset of essential RNA synthesis occurs 8 to 9 hr after virus infection and prior to the biosynthesis of other known virus-induced macromolecules.

MeSH Terms
Adenoviridae/growth & development Antimetabolites/pharmacology Culture Techniques DNA, Viral/analysis,biosynthesis Fluorouracil/pharmacology HeLa Cells Nucleosides/pharmacology Phosphorus Isotopes RNA/biosynthesis Virus Cultivation
Chemicals
Antimetabolites DNA, Viral Nucleosides Phosphorus Isotopes RNA Fluorouracil
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Flanagan J F
Ginsberg H S
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38 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.87.5.977-987.1964
Published
1964-05-00
Pages
977-87
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC277134
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.87.5.977-987.1964 PMC Full Text View journal details
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