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PMID: 58995 Published · ppublish English Journal Article

The use of ionophores of rapid loading of human red cells with radioactive cations for cation-pump studies.

The Journal of membrane biology ·Vol. 26 ·No. 4 ·1976-05-00 ·Pages 357-70

Sarkadi B, Szász I, Gárdos G

Abstract

Techniques are described for the rapid loading of intact human red cells with radioactive isotopes of alkali cations or Ca2+ by using ionophorous compounds (nigericin, gramicidin D and A 23187). Loading was rapid and efficient if the membrane potential of the cells was rendered more negative inside. After cation loading the ionophores could be bound to albumin and removed by repeated washings. The ATP and 2,3-DPG contents of the cells were practically unaltered by this treatment. Passive membrane permeability to Na+ and Ca2+ returned to normal. Loaded erythrocytes pumped out Na+ in a ouabain-sensitive and Ca2+ in a lanthanum-sensitive way. Ca2+ -loaded red cells were microspherocytes and exhibited a rapid K+ -efflux. Parallel with the extrusion of Ca2+ cells regained their biconcave shape and normal passive permeability to K+.

MeSH Terms
Adenosine Triphosphate/blood Biological Transport, Active Calcimycin/pharmacology Calcium/blood Chlorobutanol/pharmacology Diphosphoglyceric Acids/blood Erythrocytes/drug effects,metabolism,ultrastructure Gramicidin/pharmacology Humans Ionophores/pharmacology Kinetics Microscopy, Phase-Contrast Nigericin/pharmacology Oligomycins/pharmacology Ouabain/pharmacology Potassium/blood Propranolol/pharmacology Sodium/blood
Chemicals
Diphosphoglyceric Acids Ionophores Oligomycins Gramicidin Calcimycin Ouabain Adenosine Triphosphate Sodium Propranolol Chlorobutanol Nigericin Potassium Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sarkadi B
Szász I
Gárdos G
References (25)
25 references, click to expand
  1. Factors controlling the resealing of the membrane of human erythrocyte ghosts after hypotonic hemolysis.
    J Membr Biol. 1972;8(1):1-26 PMID: 4628383
  2. Sodium and potassium movements in human red cells.
    J Physiol. 1956 Nov 28;134(2):278-310 PMID: 13398911
  3. Nature of the calcium dependent potassium leak induced by (+)-propranolol, and its possible relevance to the drug's antiarrhythmic effect.
    Br J Pharmacol. 1972 Feb;44(2):271-8 PMID: 4668594
  4. Cation loading of red blood cells.
    J Physiol. 1967 Nov;193(2):459-66 PMID: 6065891
  5. Ion movements in red cells treated with propranolol.
    Acta Physiol Scand. 1969 Mar;75(3):333-44 PMID: 4389297
  6. The rate of sodium extrusion from human erythrocytes.
    J Physiol. 1953 Sep;121(3):470-86 PMID: 13097387
  7. The connexion between active cation transport and metabolism in erythrocytes.
    Biochem J. 1965 Oct;97(1):214-27 PMID: 16749106
  8. The behaviour of the sodium pump in red cells in the absence of external potassium.
    J Physiol. 1967 Sep;192(1):159-74 PMID: 6051801
  9. Equilibrium dialysis of ions in nystatin-treated red cells.
    Nat New Biol. 1973 Jul 11;244(132):47-9 PMID: 4515993
  10. ATP-dependent Ca++-extrusion from human red cells.
    Experientia. 1966 Jun 15;22(6):364-5 PMID: 5961668
  11. Determination of the stoichiometry of the calcium pump in human erythrocytes using lanthanum as a selective inhibitor.
    FEBS Lett. 1975 Feb 1;50(2):135-9 PMID: 122940
  12. Studies on the active transport of calcium in human red cells.
    J Gen Physiol. 1969 Dec;54(6):713-29 PMID: 5357189
  13. Active calcium and strontium transport in human erythrocyte ghosts.
    J Gen Physiol. 1969 Mar;53(3):311-22 PMID: 4304202
  14. The calcium content of human erythrocytes.
    J Physiol. 1968 Dec;199(2):367-81 PMID: 4972779
  15. Studies on a Ca 2+ -dependent ATPase of human erythrocyte membranes. Effects of Ca 2+ and H + .
    Biochim Biophys Acta. 1972 May 9;266(2):361-75 PMID: 4261006
  16. Red blood cell calcium and magnesium: effects upon sodium and potassium transport and cellular morphology.
    Biochim Biophys Acta. 1974 May 30;352(1):97-116 PMID: 4854055
  17. Effects of an ionophore, A23187, on the surface morphology of normal erythrocytes.
    Am J Pathol. 1974 Dec;77(3):507-18 PMID: 4432916
  18. Ion movements in human red cells independent of the sodium pump.
    J Physiol. 1969 May;202(1):111-31 PMID: 4238987
  19. Preparation and properties of human erythrocyte ghosts.
    Mol Cell Biochem. 1973 Dec 15;2(2):197-218 PMID: 4272551
  20. Dependence on calcium concentration and stoichiometry of the calcium pump in human red cells.
    J Physiol. 1973 Dec;235(2):551-69 PMID: 4271735
  21. The lanthanides Ho3+ and Pr3+ as inhibitors of calcium transport in human red cells.
    Experientia. 1971 Jan 15;27(1):59-61 PMID: 4251422
  22. A23187: a divalent cation ionophore.
    J Biol Chem. 1972 Nov 10;247(21):6970-7 PMID: 4263618
  23. Calcium movements across the membrane of human red cells.
    J Physiol. 1969 Apr;201(2):369-95 PMID: 4238381
  24. The effect of 2,4,6-trinitro-m-cresol on cation and anion transport in sheep red blood cells.
    J Gen Physiol. 1971 May;57(5):593-609 PMID: 5553103
  25. Ion transport across the erythrocyte membrane.
    Haematologia (Budap). 1972;6(3):237-47 PMID: 4150656
Article Info
Journal
The Journal of membrane biology
Abbr.
J Membr Biol
ISSN
0022-2631
DOI
10.1007/BF01868883
Published
1976-05-00
Pages
357-70
Language
English
Region
United States
NLM ID
0211301
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01868883 View journal details
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