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PMID: 5922546 Published · ppublish English Journal Article

Isolation and morphology of temperature Agrobacterium tumefaciens bacteriophage.

Journal of bacteriology ·Vol. 92 ·No. 3 ·1966-09-00 ·Pages 746-50

Zimmerer RP, Hamilton RH, Pootjes C

Abstract

Zimmerer, Robert P. (The Pennsylvania State University, University Park), Robert H. Hamilton, and Christine Pootjes. Isolation and morphology of temperate Agrobacterium tumefaciens bacteriophage. J. Bacteriol. 92:746-750. 1966.-Lysogeny was detected in 14 strains of Agrobacterium tumefaciens among 130 bacterial strains tested with strain B-6 used as the host. Partial lysis was observed with 13 additional bacterial strains. Morphological studies of five strains showed that the phage had similar features. A typical phage (Lv-1) had a polyhedralshaped head, approximately 71 by 63 mmu, and a tail, approximately 211 mmu by 9.5 mmu. The phage nucleic acid was found to be deoxyribonucleic acid. The bacteriophage have been designated L (for lysogenic) followed by the bacterial strain designation in lower case letters.

MeSH Terms
Bacteriophage Typing Bacteriophages/cytology,isolation & purification Chemical Phenomena Chemistry DNA, Viral/analysis Rhizobium Spectrum Analysis
Chemicals
DNA, Viral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Zimmerer R P
Hamilton R H
Pootjes C
References (6)
6 references, click to expand
  1. Agrobacterium tumefaciens Conn. I. Release of P32 and S35 by labeled bacteria in vitro.
    J Bacteriol. 1960 Jun;79:880-8 PMID: 13834942
  2. Agrobacterium tumefaciens Conn. II. Production of an antibiotic substance.
    J Bacteriol. 1960 Jun;79:889-98 PMID: 13834943
  3. The nucleic acids of plant tissues; the extraction and estimation of desoxypentose nucleic acid and pentose nucleic acid.
    Arch Biochem. 1950 Feb;25(2):262-76 PMID: 15404816
  4. Lysogenicity in Agrobacterium tumefaciens.
    J Bacteriol. 1960 Aug;80:180-7 PMID: 13855543
  5. GROWTH CHARACTERISTICS OF PHAGES OF AGROBACTERIUM RADIOBACTER.
    Can J Microbiol. 1965 Feb;11:95-101 PMID: 14290964
  6. A negative staining method for high resolution electron microscopy of viruses.
    Biochim Biophys Acta. 1959 Jul;34:103-10 PMID: 13804200
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
Published
1966-09-00
Pages
746-50
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC276318
Subset
IM
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