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PMID: 5931920 Published · ppublish English Journal Article

Antinuclear antibodies in rabbit antisera.

The Journal of experimental medicine ·Vol. 123 ·No. 4 ·1966-04-01 ·Pages 733-46

Barnett EV, Vaughan JH

Abstract

Antinuclear antibodies were detected by immunofluorescence in most sera from rabbits immunized with whole human serum emulsified in Freund's complete adjuvant. Four of 14 sera from rabbits immunized with Cohn fractions III, IV-1, or IV-4 also gave positive nuclear fluorescence with human leukocyte nuclei. Other human and animal nuclei gave negative results with these rabbit antisera. Three rabbit anti-whole human sera had complement-fixing antibody against DNA in sufficient titer for study in quantitative complement fixation tests. Antibody with greatest reactivity with human single strand DNA was detected in the 3 rabbit antisera. Reactivity with rabbit, calf thymus, and B. natto DNA was also detected. In each case reactivity was greater with single strand than with native DNA. Antibodies against human histone and purine-6-oly BSA were also detected. No correlation was found between the titers of rheumatoidlike factors and antinuclear antibodies present in the rabbit antisera. The induction of antinuclear antibodies in these rabbits was not associated with disease attributable to the antibodies. The induction of antinuclear antibody by immunization with whole human serum was interpreted as indicating the presence of antigenic nuclear material in whole human serum.

MeSH Terms
Animals Antibodies Blood Cattle Cell Nucleus Complement Fixation Tests DNA Deoxyribonucleases Histones Humans Immune Sera Immunochemistry In Vitro Techniques Leukocytes Mercaptoethanol Microscopy, Fluorescence Purines Rabbits Ribonucleases Serum Albumin, Bovine Trypsin
Chemicals
Antibodies Histones Immune Sera Purines Serum Albumin, Bovine Mercaptoethanol DNA Deoxyribonucleases Ribonucleases Trypsin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Barnett E V
Vaughan J H
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20 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.123.4.733
Published
1966-04-01
Pages
733-46
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2180463
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.123.4.733 PMC Full Text View journal details
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