Purification and properties of rabbit uterus preuteroglobin mRNA.

Nucleic acids research ·Vol. 4 ·No. 11 ·1977-11-00 ·Pages 4023-36

Arnemann J, Heins B, Beato M

Abstract

The mRNA for preuteroglobin, a precursor of the hormonally induced protein uteroglobin, has been partially purified from the endometrium of progesterone treated rabbits. The purification procedure starts with total endometrial polysomes and involves treatment with proteinase K and dodecylsulfate, chromatography on oligo(dT)-cellulose, sucrose density gradient centrifugation, electrophoresis in polyacrylamide gels containing dodecylsulfate, and a second absorption to oligo(dT)-cellulose. The final mRNA preparation codes exclusively for preuteroglobin in a wheat germ cell-free system and migrates as a single band in polyacrylamide gels containing 99% formanide. The average length of the poly(A) segment is 60 nucleotides and the translation of the preuteroglobin mRNA is inhibited by m7G(5')ppp(5')A, indicating that it contains a "capped" 5'-terminus. Comparison with known standards yields a molecular weight of 200,000 (600 nucleotides) for preuteroglobin mRNA, approximately twice as many nucleotides as required for encoding the 90 aminoacids of its cell-free product.

MeSH Terms

Animals Female Glycoproteins/genetics Guanosine Triphosphate/pharmacology Molecular Weight Oligonucleotides/pharmacology Poly A/analysis Polyribosomes/analysis Protein Biosynthesis/drug effects Protein Precursors/genetics RNA, Messenger/isolation & purification,metabolism Rabbits Uteroglobin/genetics Uterus/analysis

Chemicals

Glycoproteins Oligonucleotides Protein Precursors RNA, Messenger Poly A Guanosine Triphosphate Uteroglobin

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Arnemann J

Heins B

Beato M

References (24)

24 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/4.11.4023

Published

1977-11-00

Pages

4023-36

Language

English

Region

England

NLM ID

0411011

PMCID

PMC343219

Subset

External Links

PubMed source DOI: 10.1093/nar/4.11.4023 PMC Full Text View journal details

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Purification and properties of rabbit uterus preuteroglobin mRNA.

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