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PMID: 5964961 Published · ppublish English Journal Article

Clearing-factor lipase in adipose tissue. A medium in which the enzyme activity of tissue from starved rats increases in vitro.

The Biochemical journal ·Vol. 99 ·No. 3 ·1966-06-00 ·Pages 640-7

Salaman MR, Robinson DS

Abstract

1. When epididymal fat bodies from starved rats are incubated for 3.5hr. at 37 degrees in a defined medium in vitro the total clearing-factor lipase activity rises to approximately twice its initial value. 2. During the incubation period part of the tissue clearing-factor lipase activity appears in the medium. 3. Heparin, glucose, insulin, and HCO(3) (-) and K(+) ions are shown to be important medium constituents.

MeSH Terms
Adipose Tissue/enzymology Amino Acids/metabolism Animals Bicarbonates/pharmacology Culture Media Glucose/metabolism Heparin/pharmacology Insulin/pharmacology Lipid Metabolism Lipoprotein Lipase/metabolism Lipoproteins/blood Rats Starvation
Chemicals
Amino Acids Bicarbonates Culture Media Insulin Lipoproteins Heparin Lipoprotein Lipase Glucose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Salaman M R
Robinson D S
References (17)
17 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
Published
1966-06-00
Pages
640-7
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1265053
Subset
IM
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