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PMID: 598849 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Rat hepatocyte primary cell cultures. III. Improved dissociation and attachment techniques and the enhancement of survival by culture medium.

In vitro ·Vol. 13 ·No. 12 ·1977-00-00 ·Pages 809-17

Williams GM, Bermudez E, Scaramuzzino D

Abstract

The conditions for obtaining representative, adult rat hepatocyte primary cell cultures were improved such that viable yields of 50% of the liver were produced which gave rise to cultures representing 30% of the liver. The survival of these cultures in various media was compared revealing that in complex media, particularly containing galactose, survival was improved.

MeSH Terms
Animals Cell Adhesion Cell Count Cell Separation/methods Cell Survival/drug effects Cells, Cultured Culture Media Galactose/pharmacology In Vitro Techniques Liver Perfusion Rats
Chemicals
Culture Media Galactose
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Williams G M
Bermudez E
Scaramuzzino D
References (33)
33 references, click to expand
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Article Info
Journal
In vitro
Abbr.
In Vitro
ISSN
0073-5655
DOI
10.1007/BF02615128
Published
1977-00-00
Pages
809-17
Language
English
Region
United States
NLM ID
0063733
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02615128
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