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PMID: 59927 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Simian virus to (SV40) tumor-specific proteins in nucleus and plasma membrane of HeLa cells infected by adenovirus 2-SV40 hybrid virus Ad2+ND2.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 7 ·1976-07-00 ·Pages 2505-9

Deppert W, Walter G

Abstract

HeLa cells infected with the nondefective adenovirus 2-SV40 hybrid virus (Ad2+ND2) have previously been shown to synthesize two SV40-specific proteins with molecular weights of 56,000 (56 K protein) and 42,000 (42 K protein). The present study demonstrates that these proteins are immunoprecipitable with serum from hamsters bearing SV40 tumors. By peptide analysis, it is shown that the 56 K and 42 K proteins share common amino acid sequences. Both proteins are metabolically stable in nuclei and plasma membranes but unstable in the cytoplasm, as shown by pulse-chase experiments and cell fractionation studies.

MeSH Terms
Adenoviridae/metabolism Antibodies, Neoplasm/analysis Cell Membrane/metabolism Cell Nucleus/metabolism Cytoplasm/metabolism Epitopes HeLa Cells Hybridization, Genetic Peptides/analysis Simian virus 40/metabolism Viral Proteins/analysis,biosynthesis,immunology
Chemicals
Antibodies, Neoplasm Epitopes Peptides Viral Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Deppert W
Walter G
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25 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.7.2505
Published
1976-07-00
Pages
2505-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC430626
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.7.2505 PMC Full Text View journal details
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