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PMID: 600265 Published · ppublish English Journal Article

Molecular cloning of fragments of bacteriophage T4 DNA.

Molecular & general genetics : MGG ·Vol. 156 ·No. 2 ·1977-11-14 ·Pages 203-14

Wilson GG, Tanyashin VI, Murray NE

Abstract

Non-glucosylated T4 DNA was digested with R.EcoRI and the resulting fragments covalently joined to lambda vectors. The genetic content of each lambda-T4 hybrid was determined by marker-rescue tests. The isolation of many recombinants containing partial-digestion products of T4 DNA provided the overlapping sequences necessary to order fragments within the T4 genome. The present analyses include parts of the "early" region between genes 42 and 46, and much of the "late" region between genes 50 and 29. T4 cytosine-DNA digested to completion by R.EcoRI was used to identify the fragments of DNA within the lambda-T4 recombinants. The T4 cytosine-DNA was also sensitive to R.HindIII and R.Xho but not to R.BamH1.

MeSH Terms
Chromosome Mapping Coliphages/genetics Cytosine/metabolism DNA Restriction Enzymes DNA, Recombinant DNA, Viral Genes, Viral
Chemicals
DNA, Recombinant DNA, Viral Cytosine DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Wilson G G
Tanyashin V I
Murray N E
References (34)
34 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00283493
Published
1977-11-14
Pages
203-14
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00283493
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