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PMID: 600796 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A critical comparison of commonly used procedures for the assay of terminal deoxynucleotidyl transferase in crude tissue extracts.

Nucleic acids research ·Vol. 4 ·No. 12 ·1977-12-00 ·Pages 4305-12

Coleman MS

Abstract

Terminal deoxynucleotidyl transferase (TdT) is a non-template directed DNA polymerase normally found in vertebrate thymus and bone marrow. Quantitative assay of TdT activity is being widely used as a tool in the differential diagnosis of acute leukemias in man. Clinical specimens of blood and bone marrow often contain 10(7) or fewer cells and require a specific and rapid assay for transferase which can be carried out in crude cell extracts. Commonly used assay methods do not meet these requirements, but can be easily modified to do so.

MeSH Terms

Cell Line DNA DNA Nucleotidyltransferases/analysis Kinetics Methods Templates, Genetic

Chemicals

DNA DNA Nucleotidyltransferases

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Coleman M S

References (15)

15 references, click to expand

THE ENZYMATIC CONDENSATION OF OLIGODEOXYRIBONUCLEOTIDES WITH POLYDEOXYRIBONUCLEOTIDES.
Proc Natl Acad Sci U S A. 1964 Dec;52:1482-8 PMID: 14243522
Terminal deoxynucleotidyl transferase measurements in the differential diagnosis of adult leukaemias.
Br J Haematol. 1976 Nov;34(3):447-56 PMID: 62584
Terminal deoxynucleotidyl transferase: characterization of extraction and assay conditions from human and calf tissue.
Arch Biochem Biophys. 1977 Aug;182(2):525-32 PMID: 268955
DNA-dependent DNA polymerases I and II from normal human-blood lymphocytes.
Proc Natl Acad Sci U S A. 1972 Oct;69(10):2879-84 PMID: 4342965
Is terminal deoxynucleotidyl transferase a somatic mutagen in lymphocytes?
Nature. 1974 Mar 29;248(447):409-11 PMID: 4545077
Terminal deoxynucleotidyl transferase in a case of childhood acute lymphoblastic leukemia.
Proc Natl Acad Sci U S A. 1973 Feb;70(2):521-5 PMID: 4346893
Low molecular weight deoxyribonucleic acid polymerase from calf thymus chromatin. I. Preparation of homogeneous enzyme.
J Biol Chem. 1973 Jun 10;248(11):3789-95 PMID: 4708092
DNA polymerases in normal and leukemic human hematopoietic cells.
Blood. 1974 Jul;44(1):19-32 PMID: 4525506
Terminal deoxyribonucleotidyl transferase in human leukemia.
Proc Natl Acad Sci U S A. 1974 Nov;71(11):4404-8 PMID: 4530991
Low molecular weight deoxyribonucleic acid polymerase from rabbit bone marrow.
Biochemistry. 1972 Mar 28;11(7):1264-72 PMID: 5012978
Doxynucleotide-polymerizing enzymes of calf thymus gland. IV. Inhibition of terminal deoxynucleotidyl transferase by metal ligands.
Proc Natl Acad Sci U S A. 1970 Apr;65(4):1041-8 PMID: 4985880
Terminal deoxynucleotidyl transferase activity in human leukemic cells and in normal human thymocytes.
N Engl J Med. 1975 Apr 10;292(15):775-80 PMID: 1054106
Terminal deoxynucleotidyl transferase and human leukemia.
Res Commun Chem Pathol Pharmacol. 1975 Apr;10(4):715-24 PMID: 1153847
Low molecular weight deoxyribonucleic acid polymerase in mammalian cells.
J Biol Chem. 1971 Sep 25;246(18):5835-7 PMID: 5096096
Enzymatic synthesis of oligodeoxynucleotides.
Biochemistry. 1971 Feb 2;10(3):536-42 PMID: 5543985

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/4.12.4305

Published

1977-12-00

Pages

4305-12

Language

English

Region

England

NLM ID

0411011

PMCID

PMC343242

Subset

External Links

PubMed source DOI: 10.1093/nar/4.12.4305 PMC Full Text View journal details

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A critical comparison of commonly used procedures for the assay of terminal deoxynucleotidyl transferase in crude tissue extracts.

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